| 新城疫病毒SGM01分离株F基因多肽重复区的序列分析和初步表达 |
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| 引用本文： | 袁小远,欧阳文军,张玉霞,金维江,秦卓明.新城疫病毒SGM01分离株F基因多肽重复区的序列分析和初步表达[J].山东农业科学,2009(8):104-106. |
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| 作者姓名： | 袁小远 欧阳文军 张玉霞 金维江 秦卓明 |
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| 作者单位： | 山东省农业科学院家禽研究所,山东,济南,250023 |
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| 基金项目： | 山东省农业科学院创新基金(2006YCX024);;山东省农业科学院青年基金(2005YQ038)资助 |
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| 摘 要： | 从新城疫病毒（Newcastle disease virus，NDV）SGM01强毒分离株的F基因克隆出两段七肽重复序列（Heptad Repeat Region，HR）HR1和HR2，通过氨基酸序列分析显示：SGM01分离株的HR1区与F48E9、LaSota的同源性分别为95．2％、93．7％；HR2区与F48E9、LaSota的同源性分别为89．1％、85．5％。将HR1和HR2分别插入融合表达载体pGEX-6p-1，在大肠杆菌BL21（DE3）中表达，得到预期大小的目的蛋白。这为研究HR1和HR2的结构以及其在F蛋白融合过程中所起的作用奠定了基础。
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| 关 键 词： | 新城疫病毒 多肽重复区 序列分析 表达 |
Sequence Analysis and Expression of Peptide Repeat Region of F Gene from Isolated Newcastle Disease Virus Strain SGM01 |
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| YUAN Xiao-yuan,OUYANG Wen-jun,ZHANG Yu-xia,JIN Wei-jiang,QIN Zhuo-ming.Sequence Analysis and Expression of Peptide Repeat Region of F Gene from Isolated Newcastle Disease Virus Strain SGM01[J].Shandong Agricultural Sciences,2009(8):104-106. |
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| Authors: | YUAN Xiao-yuan OUYANG Wen-jun ZHANG Yu-xia JIN Wei-jiang QIN Zhuo-ming |
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| Institution: | Institute of Poultry Science;Shandong Academy of Agricultural Sciences;Jinan 250023;China |
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| Abstract: | Two heptad repeat fragments,HR1 and HR2,were amplified from F gene of isolated Newcastle disease virus(NDV) SGM01.The results of sequence analysis showed that the homology of HR1 with F48E9 and LaSota were 95.2% and 93.7% respectively,while those of HR2 were 89.1% and 85.5% respectively.The expected size protein was obtained by cloning HR1 and HR2 into the vector pGEX-6p-1 and expressing in E.coli BL21(DE3).These results provided a theoretical basis for the study of structure and action of HR1 and HR2 durin... |
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| Keywords: | Newcastle disease virus Peptide repeat region Sequence analysis Expression |
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