Fertility of Mares Inseminated With Frozen-Thawed Semen Processed by Single Layer Centrifugation Through a Colloid |
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Authors: | Katheryn L Cerny Sydney Hughes Juliana R Campos Robert J Coleman Mats HT Troedsson Edward L Squires |
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Institution: | 1. Department of Veterinary Science, Equine Reproduction, Maxwell H Gluck Equine Research Center, University of Kentucky, Lexington, KY;2. Department of Veterinary Science, Virology, Maxwell H Gluck Equine Research Center, University of Kentucky, Lexington, KY;3. Department of Animal and Food Sciences, University of Kentucky, 900 W.P Garrigus Building, Lexington, KY |
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Abstract: | The aim of this study was to determine whether there was an increase in pregnancy rates when frozen-thawed stallion semen was processed by single layer centrifugation (SLC) through a colloid before insemination. In addition, changes in semen parameters, including motility, were determined before and after SLC. Twenty light-horse mares (aged 3-16 years) and one Thoroughbred stallion (aged 16 years) having average fertility with fresh and cooled semen (>50% per cycle) and displaying a postthaw motility of >35% were used. Control mares were inseminated using 4- × 0.5-mL straws (200 × 106/mL) of frozen-thawed semen. Treatment mares were inseminated with 4 × 0.5 mL of frozen-thawed semen after processing by SLC. Pregnancy rates were compared using Fisher exact test, and continuous parameters were evaluated by a Student t test. The pregnancy rates at day 14 were not different for the mares inseminated with control versus SLC-processed semen, despite the difference in sperm number (171 × 106 ± 21, 59 × 106 ± 25 progressively motile sperm). After frozen-thawed semen was processed by SLC, the percentage progressively motile sperm improved (P < .05), and SLC processing resulted in a 21.8% recovery of spermatozoa. In summary, centrifugation of frozen-thawed semen through a single layer of colloid increased the percentage of motile spermatozoa, but did not improve pregnancy rates after deep horn insemination. |
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Keywords: | Stallion Spermatozoa Single Layer Centrifugation Cryopreservation |
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