| 3种脱除南瓜水溶性多糖蛋白方法的比较 |
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| 引用本文： | 李俊丽,王运强,向长萍.3种脱除南瓜水溶性多糖蛋白方法的比较[J].湖北农业科学,2008,47(6). |
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| 作者姓名： | 李俊丽 王运强 向长萍 |
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| 作者单位： | 1. 华中农业大学园艺林学学院/国家蔬菜改良中心华中分中心,武汉,430070;武汉理工大学化学工程学院,武汉,430070
2. 华中农业大学园艺林学学院/国家蔬菜改良中心华中分中心,武汉,430070 |
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| 基金项目： | 湖北省三峡移民局资助项目 |
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| 摘 要： | 探讨了三氯乙酸法、重金属离子沉淀法和Sevag法3种方法对南瓜多糖提取物进行脱除蛋白处理的效果.结果表明,综合考虑多糖损失率、脱除蛋白率和活性等因素,Sevag法优于三氯乙酸法和重金属离子沉淀法,是适用于南瓜水溶性多糖脱除蛋白的有效方法.
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| 关 键 词： | 南瓜 多糖 游离蛋白 |
Comparison of Three Methods for Removal of Protein from Pumpkin Polysaccharide |
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| LI Jun-li,WANG Yun-qiang,XIANG Chang-ping.Comparison of Three Methods for Removal of Protein from Pumpkin Polysaccharide[J].Hubei Agricultural Sciences,2008,47(6). |
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| Authors: | LI Jun-li WANG Yun-qiang XIANG Chang-ping |
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| Institution: | LI Jun-li1,2,WANG Yun-qiang1,XIANG Chang-ping1 (1.College of Horticulture , Forestry,Huazhong Agricultural University/ Huazhong Branch of National Vegetable Improvement Center,Wuhan 430070,China,2. College of Chemical Engineering,Wuhan University of Technology,China) |
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| Abstract: | In this study,three methods (trichloroacetic acid method, Cu2+ method and Sevag method) were used to remove protein from pumpkin polysaccharide. The results showed that Sevag method was better than other two methods to remove protein from pumpkin polysaccharide, which was more effective in the removal percentage of protein, the loss of polysaccharide and physiological activity of polysaccharide. |
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| Keywords: | pumpkin polysaccharide removal of protein |
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