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Identification of a diverse mini‐core panel of Indian rice germplasm based on genotyping using microsatellite markers |
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| Authors: | Kapil K. Tiwari Anshuman Singh Sasmita Pattnaik Maninder Sandhu Sukhdeep Kaur Sourabh Jain Sushma Tiwari Shweta Mehrotra Mahender Anumalla Rashmita Samal Jyoti Bhardwaj Neha Dubey Vikrant Sahu Gayle A. Kharshing Patu K. Zeliang Kadiri Sreenivasan Pankaj Kumar Swarup K. Parida Sevanthi V. A. Mithra Vandana Rai Wricha Tyagi Pawan K. Agrawal Atmakuri R. Rao Arunava Pattanayak Girish Chandel Ashok K. Singh Ishwari S. Bisht Kangila V. Bhat Gundimeda J. N. Rao Jitendra P. Khurana Nagendra K. Singh Trilochan Mohapatra |
| Affiliation: | 1. National Research Centre on Plant Biotechnology, New Delhi, India;2. Central Rice Research Institute, Cuttack, Odisha, India;3. National Bureau of Plant Genetic Resources, New Delhi, India;4. Division of Genetics, Indian Agricultural Research Institute, New Delhi, India;5. Indira Gandhi Krishi Vishwavidyalaya, Raipur, India;6. Central Agriculture University, Barapani, Meghalaya, India;7. ICAR Research Complex for North Eastern Hill Region, Barapani, Meghalaya, India;8. Directorate of Rice Research, Hyderabad, India;9. Vivekanand Parvatiya Krishi Anusandhan Sansthan, Almora, Uttrakhand, India;10. Indian Agricultural Statistics Research Institute, New Delhi, India;11. Delhi University South Campus, New Delhi, India |
| Abstract: | Identification of a small core germplasm set representing the available genetic diversity is essential for its proper evaluation and subsequent utilization in rice improvement programmes. For constituting a small diverse mini‐core panel of Indian rice germplasm, a representative set of 6912 accessions drawn based on their geographic origin from the whole rice germplasm collection available in the National Gene Bank was genotyped using 36 microsatellite markers. Automated fragment analysis of amplicons yielded a total of 435 alleles, with an average 12.4 and range of 3–29 alleles per locus. Polymorphism information content (PIC) ranged from 0.08 (RGNMS190) to 0.86 (RM552) with an average of 0.528. Based on genotyping data, a mini‐core consisting of 98 genotypes was identified. Ninety‐four per cent of the alleles present in the core set were present in the mini‐core. The identified small but diverse panel will be useful for further intensive trait‐specific evaluation and utilization in allele mining. |
| Keywords: | allele mining heuristic approach Indian rice germplasm mini‐core microsatellite markers |