Identification and mapping of a novel Turnip mosaic virus resistance gene TuRBCS01 in Chinese cabbage (Brassica rapa L.) |
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| Authors: | Qiaoyun Li Xiaoliang Zhang Qiang Zeng Zhigang Zhang Shuantao Liu Yuhe Pei Shufen Wang Xianxian Liu Wenling Xu Weimin Fu Zhizhong Zhao Xiyun Song |
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| Affiliation: | 1. Institute of Vegetable and Flower Research, Shandong Academy of Agricultural Sciences, National Improvement Center for Vegetables, ShanDong Branch, Key Laboratory for Biology of Greenhouse Vegetable of Shandong Province, Jinan, China;2. College of Life Sciences, Qingdao Agricultural University, Qingdao, China |
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| Abstract: | We aimed to identify Turnip mosaic virus (TuMV) resistance genes in Chinese cabbage by analysing the TuMV resistance of 43 P1 (resistant), 88 P2 (susceptible), 26 F1, 104 B1 (F1 × P1), 108 B2 (F1 × P2) and 509 F2 individuals. All parents and progeny populations were mechanically inoculated with TuMV‐C4. Both F1 and B1 populations showed TuMV resistance. Resistant: susceptible ratios in the B2 and F2 populations were 1 : 1 and 3 : 1, respectively. TuMV resistance in P1 was controlled by a dominant gene, TuRBCS01. Bulked segregation analysis was performed to identify simple sequence repeat or insertion or deletion markers linked to TuRBCS01. Data from 108 B2 individuals with resistant or susceptible phenotypes were analysed using mapmake r/exp 3.0. Polymorphic marker sequences were blast searched on http://brassicadb.org/brad/ . TuRBCS01 was found to be linked to eight markers: SAAS_mDN192117a_159 (3.3 cM), SAAS_mDN192117b_196 (4.0 cM), SAAS_mDN192403_148 (13.0 cM), SAAS_mGT084561_233 (6.8 cM), BrID10723 (3.3 cM), mBr4041 (3.3 cM), SAAS_mBr4055_194 (2.6 cM) and mBr4068 (4.0 cM). Further, TuRBCS01 was mapped to a 1.98‐Mb region on chromosome A04 between markers BrID10723 and SAAS_mBr4055_194. |
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| Keywords: | Chinese cabbage TuMV resistance gene Identification mapping |
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