复方霍山石斛含片清咽功能的动物试验研究

目的]研究复方霍山石斛含片是否具有清咽功能。方法]根据国食药监保化2012]107号文件清咽功能动物试验要求,采用大鼠棉球植入、大鼠足趾肿胀、小鼠耳廓肿胀3种试验方法。具体将80只健康雄性大鼠、40只健康雄性小鼠按体重随机分2个大组,每个大组按低、中、高和空白对照4个剂量组0.27、0.53、1.60、0 g/(kg·bw·d)],连续灌胃32 d后分别对不同剂量组进行棉球植入试验(肉芽肿的形成及测定)、足趾肿胀试验及测定、耳肿胀试验及测定。结果]1.60 g/(kg·bw·d)组肉芽肿净量低于0 g/(kg·bw·d)组;0.53、1.60 g/(kg·bw·d)组1、2、4 h足趾肿胀率均低于0 g/(kg·bw·d)组,1.60 g/(kg·bw·d)组6 h足趾肿胀率低于0g/(kg·bw·d)组;1.60 g/(kg·bw·d)组耳廓肿胀率低于0 g/(kg·bw·d)组,差异均有统计学意义(P﹤0.05)。结论]复方霍山石斛含片清咽功能的动物试验结果为阳性,具有清咽功能。

Research on Throat Clearing Function of the Tablet of Dendrobium huoshanense

To study the effect of the tablet of Dendrobium huoshanense on the throat clearing function of mice. Method]The mice were fed with the tablet at the dose of 0,0. 27,0. 53 and 1. 60 for 32 days,and then 3 indexes of throat clearing function were measured to judge the effect of the tablet on clearing the throat function. Result] The net amount of granuloma of 1. 60 g /（ kg·bw·d） group is less than 0g /（kg·bw·d） group; The toe swelling rate of group 0. 53 g /g（kg·bw·d） and 1. 60 g /g /（kg·bw·d） in 1 h,2 h,4 h were lower than that of 0 g /（kg·bw·d） group; The auricle swelling rate of 1. 60 g /（kg·bw·d） group is lower than that of 0 g /（kg·bw·d） group. The differences all have statistical significance（P 0. 05）. Conclusion]The tablet has throat clearing function.