| 白花与紫花丁香ISSR-PCR鉴别研究 |
| |
| 引用本文： | 思彬彬,赵海燕,刘海涛.白花与紫花丁香ISSR-PCR鉴别研究[J].安徽农业科学,2012,40(32):15579-15580. |
| |
| 作者姓名： | 思彬彬 赵海燕 刘海涛 |
| |
| 作者单位： | 北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021;北方民族大学生物科学与工程学院,宁夏银川,750021 |
| |
| 摘 要： | 目的]探索用ISSR分子标记方法在核酸分子水平上鉴别白花与紫花丁香。方法]从100条ISSR引物中筛选合适的引物对白花和紫花丁香2个样品进行PCR扩增及电泳分析,寻找特征位点。结果]有3条ISSR引物扩增出较为明显的多态性特征条带,可单独应用于白花和紫花丁香的鉴别。结论]ISSR作为一种简便、可靠的分子标记方法,可用于不同花色丁香的鉴别。
|
| 关 键 词： | 丁香 ISSR 鉴别 |
Identification of Syringa oblata by Inter-Simple Sequence Repeat Markers |
| |
| Institution: | SI Bin-bin et al(College of Biological Science and Engineering,Beifang University for Nationalities,Yinchuan,Ningxia 750021) |
| |
| Abstract: | Objective] To identify Syringa oblata by inter-simple sequence repeat markers.Method] Primers suitable for routine analysis were screened from 100 inter-simple sequence repeat primers,then,they were used in PCR and separated of 2 samples of Syringa oblata.Results] Three of the one-hundred primers amplified polymorphic bands and suitable for the identification of Syringa oblata.Conclusion] Inter-simple sequence repeat markers provide a quick,reliable molecular marker for identification of Syringa oblata. |
| |
| Keywords: | Syringa oblata ISSR Identification |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
