|
|||||
|
|
| 大鼠GTH细胞PKC对FSH-β mRNA表达的影响 | |
| 引用本文： | 王新,谭建华,赖小平.大鼠GTH细胞PKC对FSH-β mRNA表达的影响[J].动物医学进展,2012,33(4):45-48. |
| 作者姓名： | 王新 谭建华 赖小平 |
| 作者单位： | 1. 青岛农业大学动物科学技术学院,山东青岛266109;广州中医药大学中药学院,广东广州510006 2. 解放军军事医学科学院十一研究所,吉林长春,130062 3. 广州中医药大学中药学院,广东广州,510006 |
| 基金项目： | 国家自然科学基金，青岛农业大学高层次人才科研基金，广州中医药大学博士后研究人员科研资助 |
| 摘 要： | 为研究大鼠促卵泡素(FSH)分泌的受体后信号转导机制,将GTH细胞用PMA或H7处理后,用GnRH脉冲刺激,再用实时荧光定量PCR方法测定细胞FSH-βmRNA表达的Ct值,并与空白对照组比较。结果表明,PMA能显著降低GTH细胞FSH-βmRNA表达的Ct值,H7则曾能显著升高,说明GTH细胞PKC活性显著影响FSH-βmRNA的表达,FSH-βmRNA的表达随着PKC活性的升高而显著升高,随着PKC活性的降低而显著降低。因此,PKC-Ca2+是GnRH脉冲刺激所引起的FSH-βmRNA表达的受体后的信号转导途径。 |
| 关 键 词： | 促性腺激素释放激素 促卵泡激素 蛋白激酶C 促性腺激素 信号转导 |
Effects of PKC in Rats' GTH Cells on the Expression of FSH-β mRNA |
|
| WANG Xin , TAN Jian-hua , LAI Xiao-ping.Effects of PKC in Rats' GTH Cells on the Expression of FSH-β mRNA[J].Progress In Veterinary Medicine,2012,33(4):45-48. | |
| Authors: | WANG Xin TAN Jian-hua LAI Xiao-ping |
| Institution: | 1.Animal Science College of Qingdao Agricultural University,Qingdao,Shandong,266109,China;2.Traditional Chinese Medical Academy of Guangzhou University of Chinese Medicine,Guangzhou,Guangdong,510006,China;3.The NO.11 Institute of Military Medical Science,PLA,Changchun,Jilin,130062,China) |
| Abstract: | This article is to analyze the post-receptor signal transduction mechanism of the secretion of follicle stimulating hormone(FSH) in rats.The gonadotropic hormone(GTH) cells was stimulated by gonadotrophin hormone-releasing hormone(GnRH) after the activity of protein kinase C(PKC) in them was treated by phorbol 12-myristate 13-acetate(PMA) or 1(5-isoquinoline sulfonyl)-2-methyl piperazine dihydroch loride(H7).Ct of FSH-β mRNA was detected by real time quantitative PCR(RTqPCR),which was compared with the control group.The result showed that PMA can significantly reduce the Ct of FSH-β mRNA in GTH cells,while H7can significantly improveit.The activity of PKC in GTH cells influences the expression of FSH-β mRNA significantly,the expression of FSH-β mRNA rises with the rise of PMA and falls with the falling of H7.The conclusion is drawn that PKC-Ca 2+ is the post-receptor signal transduction pathway that the cells stimulated by GnRH impulse express FSH-β mRNA. |
| Keywords: | gonadotrophin hormone-releasing hormone follicle stimulating hormone protein kinase C gonadotropic hormone signal transduction |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |