RAPD variability in rice (Oryza sativa L.) plants derived from desiccation-tolerant calli |
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Authors: | Dinh Thi Phong Le Thi Muoi Le Tran Binh |
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Institution: | (1) Institute of Biotechnology (IBT), National Center for Natural Science and Technology (NCST), Hoang Quoc Viet street, Cau Giay, Hanoi, Vietnam |
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Abstract: | Genetic modification from selfed progenies of 18 rice (Oryza sativa L.) plants regenerated from callus tissues which survived desiccation, were investigated at the DNA level using the random
amplified polymorphic DNA (RAPD) method. Twelve 10-mer random primers were used to amplify DNA of progenies from the regenerated
plants, and a total of 228 PCR products and 1780 DNA fragments were obtained by primers, generating between four to thirteen
major bands. The size of the amplified fragments ranged from 0.2 to 2.55 kb. The results showed that 10 out of 12 primers
produced polymorphic bands, two primers (RA31 and RA185) showed no polymorphism among plants tested. A dendrogram of the genetic
distance was constructed based on their polymorphism, demonstrating that somaclonal variation exists in rice plants regenerated
from callus which survived the desiccation treatment. Part of this variation can be useful in rice breeding.
This revised version was published online in August 2006 with corrections to the Cover Date. |
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Keywords: | desiccation genetic distance Oryza sativa L RAPD somaclonal variation |
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