| 耐辐射异常球菌K+吸收蛋白ktrA基因功能研究 |
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| 引用本文： | 鲜先毅,江世杰,崔广艳,刘盈盈,代其林,陈明,王劲.耐辐射异常球菌K+吸收蛋白ktrA基因功能研究[J].中国农业科技导报,2014,16(1):76-81. |
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| 作者姓名： | 鲜先毅 江世杰 崔广艳 刘盈盈 代其林 陈明 王劲 |
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| 作者单位： | (1.西南科技大学生命科学与工程学院, 四川 绵阳 621010,2.中国农业科学院生物技术研究所, 北京 100081) |
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| 基金项目： | 国家973计划项目(2013CB733903);国家863计划项目(2012AA063503);国家转基因生物新品种培育重大专项(2014ZX0801201B);农业部公益性行业科研专项(201103007);西南科技大学博士研究基金(11ZX7104)资助。 |
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| 摘 要： | 耐辐射异常球菌(D. radiodurans R1)ktrA基因(DR_1666)编码的K+吸收蛋白在盐胁迫和渗透胁迫中起着重要作用。采用QRT\|PCR分析表明在盐和D\|山梨糖醇胁迫条件下，野生型菌株中ktrA基因表达均显著上调。同时采用融合PCR方法和同源重组技术，构建ktrA基因缺失突变株(ΔktrA)，以野生型菌株DR做对照，分别用不同浓度的氯化钾和D\|山梨糖醇对突变株进行胁迫处理。结果显示，突变株ΔktrA对氯化钾和D\|山梨糖醇的敏感性明显高于野生型，表明ktrA基因在菌株耐受高盐和高渗透胁迫时起重要作用。该结果为深入研究D. radiodurans R1菌株中ktrA基因的功能提供了很好的基础。
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| 关 键 词： | 耐辐射异常球菌 ktrA基因 K+吸收蛋白 融合PCR 非生物胁迫 |
Functional Analysis of ktrA Gene Encoding Potassium Uptake Protein from Deinococcus radiodurans |
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| XIAN Xian\|yi,JIANG Shi\|jie,CUI Guang\|yan,LIU Ying\|ying,DAI Qi\.Functional Analysis of ktrA Gene Encoding Potassium Uptake Protein from Deinococcus radiodurans[J].Journal of Agricultural Science and Technology,2014,16(1):76-81. |
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| Authors: | XIAN Xian\|yi JIANG Shi\|jie CUI Guang\|yan LIU Ying\|ying DAI Qi\ |
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| Institution: | (1.Life Science and Engineering College, Southwest University of Science and Technology, Sichuan Mianyang 621010|2.Biotechnology Research Institute, Chinese Academy of Agricultural Sciences, Beijing 100081, China) |
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| Abstract: | The Deinococcus radiodurans R1 potassium uptake protein encoded by ktrA gene(DR\|166), played an important role under salt and osmotic stress. The quantitative real\|time PCR assay showed that the expression of ktrA gene was up\|regulated remarkably under salt and D\|sorbitol in wild strain. Construction of D. radiodurans R1 mutants deleted ktrA gene (ΔktrA) by means of fusion PCR and homologous genetic technology. Survival rates of the ΔktrA obtained and wild strain were investigated under different concentrations of KCl and D\|Sorbitol. The results indicated that the ΔktrA were much more sensitive to KCl and D\|Sorbitol treatment comparing to that of wild type, which indicated KtrA protein played an important role when influenced by salt and osmotic stress.This research will be helpful to study the functions of ktrA gene. |
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| Keywords: | D radiodurans R1 ktrA potassium uptake protein fusion PCR abiotic stress |
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