影响妊娠伊犁马结合雌激素浓度的因素分析

试验通过分析伊犁母马妊娠时间、年龄和尿量对伊犁马孕马尿中结合雌激素浓度的影响，以期为企业生产动物结合雌激素提供参考。选取相同饲养条件下妊娠期为28～38周伊犁孕马35匹并按年龄分为3组，每周采尿样1次并记录24 h尿量。高效液相色谱（HPLC）法测定结合雌激素的浓度。采用SAS 8.1最小二乘法分析妊娠时间、年龄因素对结合雌激素浓度的影响；相关分析法分析尿量与结合雌激素浓度的相关性。结果显示，妊娠时间对结合雌激素浓度有极显著影响（P＜0.01）。年龄对17α-双氢马烯雌酮硫酸钠浓度影响不显著（P＞0.05）；年龄对雌酮硫酸钠浓度和马烯雌酮硫酸钠浓度影响极显著（P＜0.01）。尿量与17α-双氢马烯雌酮硫酸钠和马烯雌酮硫酸钠浓度呈极显著负相关（P＜0.01）；尿量与雌酮硫酸钠浓度呈不显著负相关（P＞0.05）。结果表明，伊犁孕马妊娠时间是影响结合雌激素浓度的关键因素；采集尿液的伊犁孕马年龄不宜超过13岁；伊犁孕马尿液中结合雌激素浓度与孕马尿量呈极显著负相关。

Factors Analysis Influencing Conjugated Estrogens Concentration of Pregnant Yili Horse

In this study, effects of horse pregnancy time，age and urine volume on concentrations of conjugated estrogen of Yili horse were analyzed, and the results would provide the basis for animal conjugated estrogen production. Total 35 pregnant Yili horses with same rearing conditions were divided by horse age into 3 groups. Within the 28 to 38 weeks of horse pregnancy, urine samples from Yili horse were collected dividedly and urine volumes of 24 h were recorded weekly. Concentrations of conjugated estrogens in the samples were determined by HPLC. Effects of the pregnancy time and horse age on concentrations of conjugated estrogens were statistic analyzed by least squares analysis process with SAS 8.1. Correlation between urine volume and concentrations of conjugated estrogens was determined by correlation analysis process.The results showed that concentration of conjugated estrogens was affected extremely significantly by pregnancy time （P＜0.01）; concentrations of sodium estrone sulfate and sodium equilin sulfate were affected extremely significantly by horse age （P＜0.01）, but concentration of sodium 17α-dihydroequilin sulfate was not affected by horse age （P＞0.05）. The correlation analysis showed that there were extremely significant negative correlations between urine volume and 17α-dihydroequilin sulfate, sodium equilin sulfate （P＜0.01）. There was no significantly negative correlation between urine volume and sodium estrone sulfate （P＜0.05）. Thus the pregnancy time was the key factor to affect concentration of conjugated estrogens. Concentrations of conjugated estrogens could be improved by collecting urine from the pregnant horse under 13 ages and extremely significantly negative correlation with urine volume.