神经生长因子对小鼠卵巢颗粒细胞增殖的影响

本研究旨在探索神经生长因子(nerve growth factor，NGF)对小鼠卵巢颗粒细胞增殖的影响。试验建立了小鼠卵巢颗粒细胞的原代培养体系，并通过免疫荧光技术对颗粒细胞进行鉴定和检测NGF及其受体在卵巢颗粒细胞上的表达，采用MTS法检测不同浓度的NGF对昆明小鼠卵巢颗粒细胞增殖的影响。卵巢颗粒细胞在10、50、100、500 ng/mL NGF作用24 h，用酶标仪测定细胞D_{490 nm}值，试验组与对照组相比均有促进增殖的影响（P<0.05），50 ng/mL NGF试验组与对照组相比差异极显著（P<0.01）。结果表明，NGF在昆明小鼠的卵巢颗粒细胞中有表达，在一定浓度范围NGF可以促进卵巢颗粒细胞的增殖。

Effects of Nerve Growth Factor on Proliferation of Mouse Ovarian Granulosa Cells

To study the effect of nerve growth factor （NGF） on proliferation of mouse ovarian granulosa cells. Primary mouse ovarian granulosa cells were cultured successfully, immunofluorescence was used to identify granulosa cells and observe the distributions of NGF and TrkA in granulosa cells, the MTS was used to analysis the different concentrations of NGF on proliferation of mouse granulosa cells. The cells were treated with NGF of different concentrations (10, 50, 100, 500 ng/mL) for 24 h. The D_{490 nm} value of the cell that was identified was determined by micro-plate reader.Compared with the control group, each treatment group promote the proliferation of granulosa cells with different degrees (P<0.05), the effect of NGF group （50 ng/mL） on the granulosa cells proliferation was the most significant (P<0.01). These results indicated that NGF could promote proliferation of mouse granulosa cells. Also,mouse ovarian granulosa cells could express NGF and TrkA.