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| 奶牛乳房炎金黄色葡萄球菌8种毒力基因的PCR检测 | |
| 引用本文： | 谢秀兰,刘溪源,王建东,范春梅,杨慧君,康晓冬. 奶牛乳房炎金黄色葡萄球菌8种毒力基因的PCR检测[J]. 中国畜牧兽医, 2014, 41(10): 47-50 |
| 作者姓名： | 谢秀兰 刘溪源 王建东 范春梅 杨慧君 康晓冬 |
| 作者单位： | 1. 宁夏农林科学院草畜工程技术研究中心, 宁夏银川 750002;2. 宁夏大学农学院, 宁夏银川 750021;3. 宁夏银川市金凤区农牧水务局, 宁夏银川 750002 |
| 基金项目： | 宁夏自然科学基金项目(NZ12255) |
| 摘 要： | 为研究奶牛乳房炎金黄色葡萄球菌(Staphylococcus aureus,SA)主要毒力因子的分布情况,本试验利用PCR对70株临床型乳房炎、55株隐性乳房炎金黄色葡萄球菌的8种主要毒力基因进行检测。结果显示,nuc、ClfA、TSST-1、PVL、Hla、Hlb、FnBPA和FnBPB 8种毒力基因在临床型乳房炎SA菌株中的检出率分别为:100.0%(70株)、100.0%(70株)、0(0株)、5.7%(4株)、100.0%(70株)、11.4%(8株)、97.1%(68株)和100.0%(70株);在隐性乳房炎SA菌株中的检出率分别为:100.0%(55株)、100.0%(55株)、0(0株)、70.9%(39株)、98.2%(54株)、9.1%(5株)、100.0%(55株)和100.0%(55株)。结果表明,nuc、ClfA、Hla、Hlb和FnBPA 5种毒力基因是引起奶牛乳房炎的SA的最主要毒力基因;PVL基因可能是引起隐性乳房炎的重要致病基因。 |
| 关 键 词： | 奶牛乳房炎 金黄色葡萄球菌 毒力基因 PCR |
| 收稿时间： | 2014-03-07 |
Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis |
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| XIE Xiu-lan,LIU Xi-yuan,WANG Jian-dong,FAN Chun-mei,YANG Hui-jun,KANG Xiao-dong. Detection of the Main 8 Virulence Genes of Staphylococcus aureus from Bovine Mastitis[J]. China Animal Husbandry & Veterinary Medicine, 2014, 41(10): 47-50 | |
| Authors: | XIE Xiu-lan LIU Xi-yuan WANG Jian-dong FAN Chun-mei YANG Hui-jun KANG Xiao-dong |
| Affiliation: | 1. Research Center of Grass and Livestock, Ningxia Academy of Agriculture and Forestry Sciences, Yinchuan 750002, China;2. School of Agriculture, Ningxia University, Yinchuan 750021, China;3. Jinfeng Bureau of Agriculture, Animal Husbandry and Water of Yinchuan, Yinchuan 750002, China |
| Abstract: | The aim of the present study was to investigate the main virulence genes properties of Staphylococcus aureus (SA) strains from bovine mastitis.For this purpose,8 kinds of virulence genes were detected from 70 isolates of clinical mastitis and 55 isolates of subclinical mastitis with PCR.The results were as follows,the detecting rates of nuc,ClfA,TSST-1,PVL,Hla,Hlb,FnBPA and FnBPB were 100.0% (70 isolates),100.0% (70 isolates),0 (0 isolate),5.7% (4 isolates),100.0% (70 isolates),11.4% (8 isolates),97.1% (68 isolates) and 100.0% (70 isolates) respectively in clinical mastitis;whereas 100.0% (55 isolates),100.0% (55 isolates),0 (0 isolate),70.9% (39 isolates),98.2% (54 isolates),9.1% (5 isolates),100.0% (55 isolates) and 100.0% (55 isolates) in subclinical mastitis. The results implied that nuc,ClfA,Hla,Hlb and FnBPA were the main virulence genes in SA isolates both in clinical mastitis and subclinical mastitis. PVL gene might be a key pathogenic gene for the cows with subclinical mastitis. |
| Keywords: | bovine mastitis Staphylococcus aureus (SA) virulence genes PCR |
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