无血清培养关中奶山羊胚胎生殖细胞的研究

山羊胚胎生殖细胞是一种来源于胎儿原始性腺的多能干细胞，建立该细胞体外稳定分离培养体系对研究山羊繁殖育种具有重要价值。本试验通过酶消化法和组织培养法分离培养关中奶山羊胚胎生殖细胞，检测无血清培养基对细胞体外增殖的影响。结果发现，该培养基可以分离得到山羊胚胎生殖细胞，细胞集落形态典型，表达AKP、Oct4、TERT及SSEA-1。经体外分化试验表明，细胞可以分化为类胚体、成纤维样细胞、成脂细胞和卵母细胞样形态。无血清培养基可以用于山羊胚胎生殖细胞的分离与培养，本试验对进一步建立山羊胚胎生殖细胞长期培养体系提供了新的参考。

Study on Serum-free Culture of Embryonic Germ Cells from Guanzhong Dairy Goat

Goat embryonic germ cells （EGCs） was a kind of pluripotent stem cells derived from the primordial gonad, which isolation and culture system in vitro has an important value for the study of goat breeding. In this assay, Guanzhong dairy goat EGCs were isolated and cultured by enzyme digestion and tissue culture methods, and the effect of serum-free culture medium on cell proliferation in vitro was detected. The results were found that the medium could be used for obtaining of goat EGCs, and the colony morphology was typical, which expressed AKP, Oct4, TERT and SSEA-1. The EGCs could also differentiate into embryoid bodies, fibroblast-like cells, lipoblast-like cells and oocyte-like morphology by differentiation test in vitro. In conclusion, these data indicated that serum-free culture medium could be used in isolation and culture of goat EGCs, and our results provided a new reference to further establish the long-term culture system of goat EGCs.