| 兰州百合高效再生体系的建立 |
| |
| 引用本文： | 崔兴林,秦新惠,刘 芬,伊万伟.兰州百合高效再生体系的建立[J].中国蔬菜,2014,1(6):44. |
| |
| 作者姓名： | 崔兴林 秦新惠 刘 芬 伊万伟 |
| |
| 作者单位： | （;酒泉职业技术学院生物工程系，甘肃酒泉 735000；;2. 甘肃省农业科学院林果花卉研究所，甘肃兰州733000） |
| |
| 基金项目： | 2012 酒泉市市列科技计划项目 |
| |
| 摘 要： | 以兰州百合新鲜鳞片为试材进行高效再生体系的研究。结果表明：兰州百合中层、内层鳞片比外层鳞片容易再生不定芽，更适于作为外植体。鳞片在MS+0.5 mg·L-1 6-BA+0.5 mg·L-1 NAA 的诱导培养基上再生频率最高，可达90%；不定芽在MS+0.8 mg·L-1 6-BA+0.05 mg·L-1 NAA 的增殖培养基上增殖率最高，平均每芽新增芽4.5 个；不定芽在MS+0.8 mg·L-16-BA+0.05 mg·L-1 NAA+0.5 mg·L-1 AC 的壮苗生根培养基上鳞茎的膨大倍数最高，生根数量较多，小苗长势优，移栽后成活率达98% 以上。
|
| 关 键 词： | 兰州百合 NAA 6-BA 再生体系 |
Establishment of Highly Efficient Regeneration System of Lanzhou Lily |
| |
| CUI Xing-Lin,QIN Xin-Hui,LIU Fen,YI Wan-Wei.Establishment of Highly Efficient Regeneration System of Lanzhou Lily[J].China Vegetables,2014,1(6):44. |
| |
| Authors: | CUI Xing-Lin QIN Xin-Hui LIU Fen YI Wan-Wei |
| |
| Institution: | （;1.Biological Engineering Department，Jiuquan Vocational and Technical College，Jiuquan 735000，Gansu，China；;2.Institute of Woods and Flowers，Gansu Province Academy of Agricultural Sciences，Lanzhou 733000，Gansu，China） |
| |
| Abstract: | Taking fresh Lanzhou lily〔Lilium. davidii Duch. var. unicolor（Hoog）Cotton〕scale as explant，this paper explored the establishment of highly efficient regeneration system．The results showed that the middle and inner layer of the scales were superior than the outer ones，and more suitable to be explant．The scales regeneration frequency reached the highest in medium of MS+0.5 mg·L-1 6-BA+0.5 mg·L-1 NAA，up to 90.0%．A small bud had the highest proliferation rate in the medium of MS+0.8 mg·L-1 6-BA+0.05 mg·L-1 NAA，with an average number of 4.5 per bud new shoots．The medium of MS+0.8 mg·L-1 6-BA+0.05 mg·L-1 NAA+0.5 mg·L-1 AC facilitated the enlargement growth of test-tube plantlets，and their survival rate was over 98% after
transplanting． |
| |
| Keywords: | Lanzhou lily NAA 6-BA Regeneration system |
|
| 点击此处可从《中国蔬菜》浏览原始摘要信息 |
| 点击此处可从《中国蔬菜》下载免费的PDF全文 |
