硫氧还蛋白对过氧化氢诱导的BRL-3A细胞损伤的保护作用

本试验利用不同浓度过氧化氢（H₂O₂）作用于BRL-3A大鼠肝细胞，用四甲基偶氮唑蓝（MTT）比色法检测BRL-3A细胞存活数量以确定H₂O₂最适损伤浓度。试验随机分为正常对照组、H₂O₂处理组和硫氧还蛋白(2.5和5 μmol/L) 预处理组，用脂质过氧化物丙二醛（MDA）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）试剂盒测定细胞的氧化应激变化。结果显示1000 μmol/L H₂O₂对BRL-3A细胞增殖具有显著的抑制作用（P<0.05）；2.5 μmol/L 硫氧还蛋白对H₂O₂损伤的BRL-3A细胞具有保护作用，能显著抑制H₂O₂诱导的BRL-3A细胞损伤产生MDA（P＜0.05），并显著增加细胞SOD及CAT的活性（P＜0.05），从而保护肝细胞。本试验结果表明，硫氧还蛋白对H₂O₂诱导BRL-3A细胞的氧化应激损伤具有保护作用。

Protective Effect of Thioredoxin on H2O2-induced Injury of BRL-3A Cells

BRL-3A cells were injured by different doses of hydrogen peroxide （H₂O₂） and the cell survival rate was assessed by methyl thiazolyl tetrazolium (MTT) assay to select the optimal concentration of H₂O₂. The BRL-3A cells were divided into normal control，H₂O₂-induced and thioredoxin （Trx） pre-treatment groups with different concentrations. The assay kits of malondialdehyde (MDA)，superoxide dismutase (SOD) and catalase (CAT) were used to detect the alterations of oxidative stress.Cell proliferation of BRL-3A was inhibited significantly by 1000 μmol/L H₂O₂ (P＜0.05). 2.5 μmol/L Trx had the protective effect on H₂O₂-induced injury of BRL-3A cells by significantly decreasing MDA contents which were induced by H₂O₂ and increasing the activities of SOD and CAT (P＜0.05), thus protecting liver cells.Trx had protective effects on BRL-3A cells induced by H₂O₂.The mechanism might be related to its antioxidant activity.