miR-155-specific siRNA enhances chemosensitivity of Burkitt lymphoma Raji cells to cytosine arabinoside by inducing apoptosis |
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Authors: | LIU Ping-ping ZHU Jin-can ZHENG Li LIU Shan-tao TAN Guang-xiao HE Dong-mei LIU Ge-xiu |
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Institution: | 1.Department of Hematology & Oncology, Tsinghua University First Hospital, Beijing 100016, China;2.Institute of Hematology, School of Medicine, Jinan University, Guangzhou 510632, China. |
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Abstract: | AIM:To investigate the effect of miR-155-specific siRNA alone or in combination with cytosine arabinoside (Ara-C) on the growth and apoptosis of Burkitt lymphoma Raji cells. METHODS:miR-155-specific siRNA and/or Ara-C were used to treat the cells. Quantitative real-time polymerase chain reaction was used to detect the expression of miR-155. The growth of the cells was analyzed by CKK-8 assay. The cell apoptosis was determined by flow cytometry. RESULTS:The miR-155 expression level of the cells transfected with miR-155 siRNA was significantly lower than that in the 2 control groups. Ara-C or miR-155 siRNA alone inhibited the growth of Raji cells in a dose-depend manner. miR-155 siRNA combined with Ara-C produced more inhibition of cell proliferation (P<0.05). After treatment for 48 h, the apoptotic rate of Raji cells in miR-155 siRNA+Ara-C group [(38.4±1.4)%] was higher than that in Ara-C group [(16.5±0.3)%] and miR-155 siRNA group [(14.6±0.3)%], with statistically significant difference (P<0.05). The expression of caspase-3 in Ara-C+miR-155 siRNA group was increased significantly as compared with Ara-C group and miR-155 siRNA group. CONCLUSION:miR-155-specific siRNA enhances the chemosensitivity of Raji cells to Ara-C by inducing apoptosis through the caspase-3 pathway. |
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Keywords: | Cytarabine miR-155 Small interfering RNA Burkitt lymphoma Apoptosis |
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