油酸和硬脂酸对奶牛乳腺上皮细胞中脂肪酸结合蛋白3表达和脂滴分泌的影响

为确定外源添加长链脂肪酸油酸和硬脂酸对奶牛乳腺上皮细胞中脂肪酸结合蛋白3（FABP3）表达的影响，本试验采用荧光定量RT-PCR检测乳脂合成相关基因的表达情况，确定油酸和硬脂酸的最佳浓度和培养时间分别为75 μmol/L 12 h和100 μmol/L 36 h。采用Western blotting和免疫荧光法检测添加油酸和硬脂酸后FABP3表达和脂滴分泌的情况，为进一步揭示FABP3在乳脂合成信号转导通路中的作用提供理论依据。

Effect of Oleic Acid and Stearic Acid on the Expression of Fatty Acid Binding Protein 3 and Secretion of Lipid Droplets in Dairy Cow Mammary Epithelial Cells

To study the effect of oleic acid and stearic acid on the expression of fatty acid binding protein 3 (FABP3) in dairy cow mammary epithelial cells, qRT-PCR was used to detect the expression of genes related with milk fat synthesis. The optimal concentrations and time of oleic acid and stearic acid were 75 μmol/L 12 h and 100 μmol/L 36 h, respectively. Western blotting and immunofluorescence were used to detect the expression of FABP3 and secretion of lipid droplets. These data provided further experimental evidence for studying the effect of FABP3 on the milk fat synthesis signal transduction pathway.