重组植物乳杆菌生物学特性比较研究

试验对植物乳杆菌(Lactobacillus plantarum,Lp)出发菌与携带绿色荧光蛋白(green fluorescent protein,GFP)标记基因的重组植物乳杆菌(Lp-GFP)生物学特性进行了详细的比较研究,以便于深入开展益生菌植物乳杆菌在动物肠道内分布和定植规律的研究。结果显示,Lp和Lp-GFP生长曲线的变化趋势基本相同,其中Lp-GFP的生长速度略有滞后;Lp和Lp-GFP最适培养温度均为37 ℃,最适初始pH均为6.5;Lp和Lp-GFP耐高温、耐人工胃液、耐人工肠液和耐胆盐的特性趋势一致;Lp和Lp-GFP的抑菌特性无显著差异(P>0.05);除了红霉素,Lp和Lp-GFP对其他药物的敏感性基本没有发生变化。综上所述,植物乳杆菌的重组对其生物学特性没有显著的影响,这为重组标记菌株的后续培养和机理研究奠定了重要基础。

Comparative Study on Biological Characteristics of Recombinant Lactobacillus plantarum

In order to further study the distribution and adhesion of Lactobacillus plantarum in animal gastrointestinal tract,biological characteristics of Lactobacillus plantarum (LP) and recombinant Lactobacillus plantarum with GFP marker gene (Lp-GFP) had been systematically compared.The results showed that the growth curve of Lp-GFP was basically the same as Lp and Lp-GFP growth speed slightly lagging behind.The optimum growth temperature of Lp and Lp-GFP were all 37 ℃ and the optimum initial pH were all 6.5.The high temperature resistance,tolerance to artificial gastric fluid and artificial intestinal fluid,tolerance to bile salt of Lp and Lp-GFP had the same tendency.There was no significant difference on anti-bacteria character of Lp and Lp-GFP (P>0.05).The sensitivity to drugs of Lp and Lp-GFP had no differences except erythromycin.In conclusion,recombination has no significant effect on biological characteristics of Lactobacillus plantarum.These results laid an important foundation for cultivation and mechanism study of reconstructed Lactobacillus plantarum.