不同刺激剂PHA和ConA对绒山羊淋巴细胞有丝分裂中期化的影响

试验旨在探索使较高比例的淋巴细胞富集在有丝分裂G2/M期的最佳条件。运用植物血凝素(PHA)和刀豆蛋白A(ConA)对淋巴细胞进行刺激使其增殖,培养一定时间后加秋水仙素对淋巴细胞进行同步化处理,用流式细胞仪检测G2/M期的细胞数量进行比对,观察试剂的最佳作用浓度和加秋水仙素的最佳时间。结果表明,采用PHA和ConA刺激淋巴细胞增殖的最佳作用浓度是60和5 μg/mL,且淋巴细胞经ConA刺激培养45 h再加秋水仙素处理5 h G2期的比例最高为58.38%。结果提示,就绒山羊的淋巴细胞来说,ConA刺激绒山羊淋巴细胞增殖的效果比PHA好,且摸索出细胞G2/M期的时间点至关重要。

Effect of Phytohaemagglutinin and Concanavalin A in Different Stimulants on Mitosis Metaphase of Cashmere Goat Lymphocyte

This experiment was conducted to explore optimized conditions to make more Cashmere goat lymphocyte which had been treated in G2/M. Phytohaemagglutinin (PHA) and concanavalin A (ConA) reagents were used to stimulate the proliferation of lymphocytes,the colchicine reagent were used at specific times of lymphocytes cell cycle synchronization and the number of the cell in G/M was measured by flow cytometry to observe the optimal concentration and action time. The results showed that the PHA and ConA optimal concentration to stimulate the proliferation of lymphocytes were 60 and 5 μg/mL. In addition,lymphocytes with ConA stimulation culture 45 h,then colchicines was used to treat 5 h.The percentage of lymphocytes in G2/M accounted for 58.38%. The results suggested that the treatment effect of ConA worked better than PHA in proliferation of lymphocytes.