Minocycline inhibits BV-2 cell activation by regulating P2X7 receptor |
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Authors: | LIU Shu-qiong YANG Lian-hong JIANG Long-yuan YE Jin-hao |
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Institution: | 1.Department of Neurology, 2Department of Emergency, Sun Yet-sen Memorial Hospital, Sun Yet-sen University, Guangzhou 510120, China. |
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Abstract: | AIM:To explore the role of P2X7 receptor in inhibition of lipopolysaccharide (LPS)-stimulated BV-2 cell activation by minocycline. METHODS:BV-2 cells were divided into 5 groups: control group, LPS group, LPS+0.1 μmol/L Mino group, LPS+1 μmol/L Mino group and LPS+10 μmol/L Mino group. The expression of P2X7 receptor was determined by real-time PCR and Western blotting. The levels of TNF-α and IL-1β in the microglia culture supernatants were measured by ELISA. The morphological changes of the cells were also observed. RESULTS:After exposed to LPS, the expression of P2X7 receptor increased in BV-2 cells at mRNA and protein levels. The concentrations of TNF-α and IL-1β in the microglia culture supernatants also increased. Meanwhile, 0.1~10 μmol/L minocycline inhibited those changes in a dose-dependent manner. CONCLUSION:Minocycline inhibits the activation of microglia. The mechanism may be related to the P2X7 receptor. |
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Keywords: | Minocycline Receptors P2X7 Tumor necrosis factor α Interleukin 1β |
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