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马山黑山羊支原体肺炎病原的鉴定
引用本文:闭炳芬,陶立,李军,黄明学,蓝显利,蓝金红,胡萍,农启文,韦志锋,秦若甫,兰美益,杨威.马山黑山羊支原体肺炎病原的鉴定[J].中国畜牧兽医,2014,41(9):249-253.
作者姓名:闭炳芬  陶立  李军  黄明学  蓝显利  蓝金红  胡萍  农启文  韦志锋  秦若甫  兰美益  杨威
作者单位:1. 广西兽医研究所, 广西南宁 530001;
2. 广西畜禽疫苗新技术重点实验室, 广西南宁 530001;
3. 马山县动物疫病预防控制中心, 广西马山 530600
基金项目:南宁市科技攻关项目(20132312);广西壮族自治区水产畜牧局科研项目(桂渔牧科072411、桂渔牧科08283-2)。
摘    要:为了了解马山黑山羊支原体肺炎的病原,给马山黑山羊支原体肺炎的防控提供依据,本研究采用形态学观察、生化特性、生长抑制、PCR扩增测序和动物致病性试验对从马山黑山羊支原体肺炎病原中分离到的菌株进行鉴定,并进行药物敏感试验。鉴定结果表明马山黑山羊支原体肺炎的病原为绵羊肺炎支原体。药物敏感试验结果表明其对壮观霉素、卡那霉素、庆大霉素、阿米卡星、林可霉素、环丙沙星、左氧氟沙星、复方新诺明、氟苯尼考高度敏感。

关 键 词:支原体肺炎  绵羊肺炎支原体  黑山羊  
收稿时间:2014-02-12

Identification of Mycoplasma pneumonia Pathogen of Mashan Black Goat
BI Bing-fen,TAO Li,LI Jun,HUANG Ming-xue,LAN Xian-li,LAN Jin-hong,HU Ping,NONG Qi-wen,WEI Zhi-feng,QIN Ruo-fu,LAN Mei-yi,YANG Wei.Identification of Mycoplasma pneumonia Pathogen of Mashan Black Goat[J].China Animal Husbandry & Veterinary Medicine,2014,41(9):249-253.
Authors:BI Bing-fen  TAO Li  LI Jun  HUANG Ming-xue  LAN Xian-li  LAN Jin-hong  HU Ping  NONG Qi-wen  WEI Zhi-feng  QIN Ruo-fu  LAN Mei-yi  YANG Wei
Institution:1. Guangxi Veterinary Research Institute, Nanning 530001, China;
2. Guangxi Key Laboratory of Animal Vaccines and New Technology, Nanning 530001, China;
3. Animal Disease Prevention and Control Center of Mashan, Mashan 530600, China
Abstract:To understand the Mycoplasma pneumonia pathogen of Mashan Black goat and provide a basis for prevention and control of Mycoplasma pneumonia of Mashan Black goat, morphological observation, biochemical characteristics, growth inhibition, PCR amplification and sequence, animal pathogenicity test were used to identify the pathogen of Mycoplasma pneumonia. The results showed that M. ovipneumoniae was the pathogen of Mycoplasma pneumonia in Mashan Black goat. Drug susceptibility test showed that M. ovipneumoniae were sensitivity to spectinomycin, kanamycin, gentamycin, amikacin, lincomycin, ciprofloxacin, levofloxacin, SMZ-TMP and florfenicol.
Keywords:Mycoplasma pneumonia  M  ovipneumoniae  Black goat  
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