Quality Assessment of a Fluorescent Quantitative Detection Kit for Clenbuterol |
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Authors: | GE Huai-li WANG Lian LI Jin-qing ZHENG Bai-qin LI Ai-jun ZHANG Jian-min DONG Li-xue |
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Institution: | 1. Tangshan Livestock and Aquatic Products Quality Monitoring Center, Tangshan 063000, China;2. Leadingnorm Biotechnology(Beijing) Co., Ltd., Beijing 100044, China |
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Abstract: | Quantitative detection of the clenbuterol in pig urine was investigated with a clenbuterol kit based on immunofluorescence chromatography and a fluorescence quantitative detector,and the sensitivity,accuracy,precision,specificity,stability of the strip were evaluated.The results showed that the quantitative curve fitting was performed by using four parameters method,the linear coefficient R2 was 0.99972.The detecting limit was up to 0.1 μg/L.The recovery rate of 3 different concentration levels were 80% to 120%.The intra assay coefficient of variation were 12.2%,11.1% and 15.2%,respectively.The cross reaction with the 4 β-agonists and 8 antibiotics was less than 0.01%.The experiment confirmed that the kit had 1 year shelf-life.The total detection process just needed 15 min. |
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Keywords: | clenbuterol fluorescent quantitative immunochromatographic method detection |
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