Abstract: | The specific primers and probe were designed according to the nucleotide sequence of porcine circovirus type 2 (PCV2) available in GenBank,CAP gene was amplified by PCR, cloned into the pMD18-T vector and screened positive plasmid standards.By optimization of reaction conditions,we established a TaqMan Real-time PCR method for detection of PCV2. The results indicated that the method was specific, and swine influenza virus (SIV), porcine reproductive and respiratory syndrome virus (PRRSV), pig pseudorabies virus (PRV) and classical swine fever virus detection results were negative. The detection limit of the assay was 4.53×102 copies/μL of plasmid DNA,100 times higher than that of the routine PCR. The standard curve displayed a linear range from 102 to 109 copies/μL and it had a good reproducibility. |