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Investigations of barley stripe mosaic virus as a gene silencing vector in barley roots and in <Emphasis Type="Italic">Brachypodium distachyon</Emphasis> and oat |
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| Authors: | Andrzej Pacak Katrin Geisler Bodil Jørgensen Maria Barciszewska-Pacak Lena Nilsson Tom Hamborg Nielsen Elisabeth Johansen Mette Grønlund Iver Jakobsen Merete Albrechtsen |
| Institution: | 1.Department of Genetics and Biotechnology, Faculty of Agricultural Sciences,Aarhus University,Frederiksberg C,Denmark;2.Department of Plant Biology and Biotechnology, Faculty of Life Sciences,University of Copenhagen,Denmark;3.Biosystems Division, Ris? National Laboratory for Sustainable Energy,Technical University of Denmark,Roskilde,Denmark;4.Department of Gene Expression,Adam Mickiewicz University,Poznan,Poland;5.Department of Agriculture and Ecology, Faculty of Life Sciences,University of Copenhagen,Frederiksberg C,Denmark;6.Department of Forest Genetics and Plant Physiology,Swedish University of Agricultural Sciences,Ume?,Sweden;7.Section for Sustainable Biotechnology, Department of Biotechnology, Chemistry and Environmental Engineering,Copenhagen Institute of Technology, Aalborg University,Ballerup,Denmark |
| Abstract: | BackgroundGene silencing vectors based on Barley stripe mosaic virus (BSMV) are used extensively in cereals to study gene function, but nearly all studies have been limited to genes expressed in leaves of barley and wheat. However since many important aspects of plant biology are based on root-expressed genes we wanted to explore the potential of BSMV for silencing genes in root tissues. Furthermore, the newly completed genome sequence of the emerging cereal model species Brachypodium distachyon as well as the increasing amount of EST sequence information available for oat (Avena species) have created a need for tools to study gene function in these species. |
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