小尾寒羊和湖羊高繁殖力候选基因BMP15的研究

以控制Romney Inverdale绵羊和Romney Hanna 绵羊高繁殖力的骨形态发生蛋白15(bone morphogenetic protein 15,BMP15)基因为候选基因,采用PCR-RFLP方法检测BMP15基因在高繁殖力绵羊品种(小尾寒羊、湖羊)以及低繁殖力绵羊品种(道赛特羊、萨福克羊)中的多态性,同时研究该基因对小尾寒羊高繁殖力的影响.结果表明,BMP15基因在小尾寒羊、湖羊、道赛特和萨福克绵羊中既没有发生与Inverdale绵羊相同的V31D突变,也没有发生与Hanna 绵羊相同的Q23Ter突变.还表明BMP15基因中影响Inverdale绵羊和Hanna 绵羊高繁殖力的突变位点对小尾寒羊和湖羊的高繁殖力没有显著影响.

Study on Bone Morphogenetic Protein 15 as a Candidate Gene for Prolificacy of Small Tail Han Sheep and Hu Sheep

Bone morphogenetic protein 15 (BMP15) gene which controls the fecundity of Inverdale ewes and Hanna ewes was studied as a candidate gene for the prolificacy of Small Tail Han sheep and Hu sheep. Polymorphism of BMP15 gene was detected in both high fecundity sheep breeds (Small Tail Han sheep and Hu sheep) and low fecundity sheep breeds (Suffolk sheep and Dorset sheep) by PCR-RFLP.The results show that both V31D mutation in Inverdale ewes and Q23Ter mutation in Hanna ewes of BMP15 gene do not exist in Small Tail Han sheep, Hu sheep, Suffolk sheep and Dorset sheep,and the mutation with significant effect on fecundity of Inverdale ewes and Hanna ewes has no significant effect on prolificacy of both Small Tail Han sheep and Hu sheep.