小麦穗发芽抑制剂—YSR的田间防治和作用机理

以穗发芽敏感品种AT265为试材，对本课题组筛选出的YSR纯天然小麦穗发芽抑制剂进行了田间防治；用3，5—二硝基水杨酸比色法，测定了YSR不同浓度处理的种子萌发期间的α–淀粉酶活性。结果表明，收获前2d喷施YSR抑制剂防治效果最佳，穗发芽抑制率可达75.9%，对AT265的种用价值无显著性影响，可作种用。用不同浓度的YSR处理通过休眠的小麦种子，随着浓度的增加，α–淀粉酶活性迅速下降，直至完全失去活性。YSR通过降低α–淀粉酶活性来达到抑制穗发芽的目的。

Studies on Field Prevention of Sprouting depressor-YSR and Its Function Mechanism

Using sprouting sensitive variety, AT265, the field prevention and cure experiment was undertaken with the natural sprouting depressor -YSR, obtained by the authors. Using colorimetric determination of 3, 5-dinitro-salicylic acid, α-amylase was tested for the seeds treated with different concentration YSR during germination. The results were as follows: the best prevention and cure time of YSR was 2d before harvesting, inhibiting rate was 75.9%, and YSR had no side effect to seed using value. α-amylase content fallen rapidly with increasing of YSR concentration. This proved that YSR could inhibit sprouting through lowering α-amylase during germination in wheat gains.