低温弱光对苗期紫花苜蓿根颈生理特性的影响

以秋眠级不同的2个紫花苜蓿Medicago sativa品种(巨人201和WL414)为试验材料,研究了其在低温(0~5℃和5~10℃)与弱光[50和100μmol/(m2.s)]互作处理条件下的生理响应。结果表明:紫花苜蓿根颈在低温弱光胁迫下启动了自身应激机制,对逆境胁迫做出了适应性反应,相同弱光条件下,低温0~5℃比5~10℃对根颈的影响显著,过氧化物酶活性上升幅度较小,而超氧化物歧化酶活性的下降和丙二醛含量的增加幅度均较大;相同低温处理,弱光50μmol/(m2.s)处理对根颈生理指标影响更为显著,过氧化物酶活性上升、超氧化物歧化酶活性下降和丙二醛含量增加幅度大于弱光100μmol/(m2.s)处理;巨人201各项指标的变化都优于WL414,表明巨人201抗寒耐弱光能力都可能优于WL414。

Effects of low temperature and weak light on physiological traits of alfafa's root crown

Total RNA from root, stem and leaf of Creeeping rooted alfalfa were extracted with four methods, including a modified CTAB method, TRNzol method and other two methods, and its quality and quantity were compared. The results indicated that the modified CTAB method was better than the original method since the value of OD260 nm/OD230 nm and OD260 nm/OD280 nm were 2.0 and 1.8, the purities were great higher, the bands of 28S and 18S rRNA on Gel eletro pH oresis were very clear and did not degradated. Comparably, TRNzol method was not very good as the first method because RNA was easy degraded and dispersed although the value is higher, and other two methods were not fit the requirement of RNA extraction.