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| 高山杜鹃RED JACK试管苗生根研究 | |
| 引用本文： | 陈平芬,王连润,高飞,孔令明,陶磅,刘家迅,百红.高山杜鹃RED JACK试管苗生根研究[J].现代农业科技,2012(24):171+177. |
| 作者姓名： | 陈平芬 王连润 高飞 孔令明 陶磅 刘家迅 百红 |
| 作者单位： | 1. 昆明金科艺花卉公司,云南昆明,650213 2. 昆明金科艺花卉公司,云南昆明650213 云南省农科院园艺研究所 3. 云南省农科院园艺研究所 |
| 基金项目： | 昆明市科技计划项目(11S010404);昆明市官渡区科技计划项目(2012-6) |
| 摘 要： | 以高山杜鹃REDJACK的无菌芽苗为材料,研究外源激素对高山杜鹃REDJACK试管苗生根的影响。结果表明：在不添加生长素的1/4MS培养基上,有3%的试管苗生根,添加IBA、NAA及IBA＋NAA复合物对试管苗生根均有明显的促进作用,理想的生根培养基是：1/4MS＋NAA1.20mg/L＋IBA1.20mg/L,添加蔗糖30g/L,活性炭10g/L,琼脂5g/L,pH值5.5,生根率达78%。 |
| 关 键 词： | 高山杜鹃 组织培养 试管生根 |
Study on Evergreen Rhododendron hybrids RED JACK Shoots Rooting in Vitro |
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| Authors: | CHEN Ping-fen WANG Lian-run GAO Fei KONG Ling-ming TAO Pang LIU Jia-xun WAN Hong |
| Institution: | 1 Kunming King-Keys Flower Limited Company,Kunming Yunnan 650213;2 Institute of Horticulture,Yunnan Academy of Agricultural Sciences) |
| Abstract: | Using evergreen Rhododendron hybrids RED JACK micro-propagation shoots as material,the test studied the effects of exogenous plant hormones on shoots rooting in vitro.The results showed that the 1/4MS medium without auxins was able to induce roots,but the rooting rate was only 3%.The rooting frequency improved significantly with the addition of IBA、NAA and IBA+NAA complexes.The optimal rooting medium was 1/4 MS+NAA 1.20 mg/L+ IBA 1.20 mg/L,adding sugar 30 g/L,activated carbon 10 g/L,agar 5 g/L,pH 5.5,in which rooting rate was 78%. |
| Keywords: | evergreen rhododendron hybrids tissue culture rooting in vitro |
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