陕西省鸡肾型传染性支气管炎病毒的分离鉴定

从临床表现呼吸道传染病的20个发病鸡群中先后分离出18株病毒,通过鸡胚感染、血清学试验、分子生物学试验、动物回归试验证明其中13株是冠状病毒科的鸡传染性支气管炎病毒,3株为新城疫病毒,2株为H9禽流感病毒。该13株中的BJ2、FF2、YL2、B01、G5、YX、WH、WG分离毒具有很强的嗜肾性和致病力,这8个陕西流行株的血清型与W118毒株相同,而与IBVGray株、W93株有一定的差异。结果表明,陕西地区主要流行鸡肾型传染性支气管炎,且流行株具有强致病力。

Isolation and Identification of Avian Nephropathogenic Infectious Bronchitis Virus from Shaanxi Province

Eighteen isolates were isolated successively from 20 suspected flocks with respiratory infection symptoms,and 13 of them were identified to be of infectous bronchitis virus(IBV),which belonged a member of the family Coronaviridae,3 of the rest were Newcastle disease virus(NDV),the last 2 were H9 subtype of avian influenza virus. By chicken embryo infection,serological test,molecular biological test and animal infection tests,the results of animal infection test showed that the 8 isolates,including BJ2,FF2,YL2,B01,G5,YX,WH and WG,had strongly nephropathogencity,moreover,the results of neutralization test in embryonated eggs revealed the control strain W118 could neutralize the 8 isolates completely,while Gray and W93 could not do that,they were able to neutralize W118 to a considerable high degree. In conclusion,the main prevalent strain of IBV in Shaanxi province was nephropathogenetic infectious bronchitis virus.