| 优系欧李茎叶愈伤组织诱导与植株再生 |
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| 引用本文： | 李劲,蒋泽平,梁珍海.优系欧李茎叶愈伤组织诱导与植株再生[J].江苏林业科技,2006,33(1):12-15. |
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| 作者姓名： | 李劲 蒋泽平 梁珍海 |
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| 作者单位： | 江苏省林业科学研究院,江苏,南京,211153 |
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| 摘 要： | 以欧李春季萌发的幼茎、叶片为外植体诱导愈伤组织的产生,结果表明:叶片愈伤组织培养以改良MS为基本培养基,附加NAA 1.0 mg/L IBA 0.5 mg/L BA 0.2 mg/L效果较好;茎段愈伤组织培养以改良MS附加2,4-D0.5 mg/L NAA 0.3 mg/L BA 0.15 mg/L诱导愈伤组织效果好;愈伤组织再诱导不定芽以1/3 MS附加BA2.0 mg/L IBA 0.01 mg/L培养基效果佳。
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| 关 键 词： | 欧李 愈伤组织 分化 芽苗 |
| 文章编号： | 1001-7380(2006)01-0012-04 |
| 收稿时间： | 2005-10-30 |
| 修稿时间： | 2005-11-15 |
Stem and leaf cali induction and plantlet regeneration of Cerasus humilis |
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| LI Jin,JIANG Ze-ping,LIANG Zhen-hai.Stem and leaf cali induction and plantlet regeneration of Cerasus humilis[J].Journal of Jiangsu Forestry Science & Technology,2006,33(1):12-15. |
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| Authors: | LI Jin JIANG Ze-ping LIANG Zhen-hai |
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| Institution: | jiangsu Academy of Forestry, Nanjing 211153, China |
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| Abstract: | Results from the stem and leaf cali induction of Cerasus humilis showed that the modified MS supplemented with(1.0 mg/L) NAA (0.5 mg/L) IBA (0.2 mg/L)BA is optimal for leaf callus induction while the modified MS with(0.5 mg/L) 2,4-D (0.3 mg/L) NAA (0.15 mg/L) BA optimal for the stem callus formation.As far as plantlet regeneration,the medium as 1/3 MS (2.0 mg/L) BA (0.01 mg/L) IBA performed the best. |
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| Keywords: | Cerasus humilis Callus Differentiatiion Shoot |
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