| 转基因水稻深加工产品两种荧光定量方法的比较研究 |
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| 引用本文： | 敖金霞,高学军,权东升,徐迪,于艳波,李璐,袁肖寒.转基因水稻深加工产品两种荧光定量方法的比较研究[J].东北农业大学学报,2011,42(1):34-39. |
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| 作者姓名： | 敖金霞 高学军 权东升 徐迪 于艳波 李璐 袁肖寒 |
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| 作者单位： | 东北农业大学生命科学与生物技术研究中心;黑龙江省家畜繁育指导站; |
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| 基金项目： | 黑龙江省青年科学基金项目(QC2009C49); 中国博士后基金项目(20100471242) |
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| 摘 要： | 根据转基因水稻中外源基因Cry1A(B)和内源基因SPS设计SYBR Green I引物、TaqMan引物和荧光探针,以内源基因SPS作为内参照,使用两种FQ-PCR方法对水稻深加工产品中转基因水稻的含量进行定量检测.建立了转基因水稻标准品Cry1A(B)和SPS之间的△Ct与样品中转基因水稻含量百分比之间的校正曲线和...
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| 关 键 词： | 转基因水稻 深加工制品 实时荧光定量PGR TaqMan SYBR Green I |
Comparison of two real-time PCR techniques for quantification of GMO contents in highly processed products of rice |
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| AO Jinxia,GAO Xuejun,QUAN Dongsheng,XU Di,YU Yanbo,LI Lu,YUAN Xiaohan.Comparison of two real-time PCR techniques for quantification of GMO contents in highly processed products of rice[J].Journal of Northeast Agricultural University,2011,42(1):34-39. |
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| Authors: | AO Jinxia GAO Xuejun QUAN Dongsheng XU Di YU Yanbo LI Lu YUAN Xiaohan |
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| Institution: | AO Jinxia1,GAO Xuejun1,QUAN Dongsheng2,XU Di2,YU Yanbo1,LI Lu1,YUAN Xiaohan1(1.Life Science and Biotechnique Research Center,Northeast Agricultural University,Harbin 150030,China,2.Livestock Breeding Station Guide of Heilongjiang Province,Harbin 150060,China) |
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| Abstract: | Transgenic rice was quantitatively detected by ABI Prism 7300 sequence detector with the PCR primers and fluorescence probes designed according to the sequences of endogenous SPS gene and exogenous Cry1A(B) gene,and the PCR systems were based on SYBR Green Ⅰ and TaqMan.The standard curve of △Ct between Cry1A(B) gene and SPS gene of transgenic rice in standard materials were generated and a linear regression equation was obtained.Quantification methods were optimized through two different real-time PCR chemistries,i.e.SYBR Green Ⅰ and TaqMan,and the transgenic rice contents were quantified in five standard samples and four highly processed products by two assays.Both methods are proved to be specific,highly sensitive and reliable for both identification and quantification of rice DNA.The results showed that the two optimized PCR systems could be used for practical quantitative detection of transgenic rice in highly processed products. |
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| Keywords: | TaqMan SYBR Green I |
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