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小麦网腥黑穗病温室内人工接种方法的探索
引用本文:秦丹丹,王 俊,高海峰,李宁静,吴 伟,刘太国,陈万权,高 利.小麦网腥黑穗病温室内人工接种方法的探索[J].植物保护,2021,47(1):165-168.
作者姓名:秦丹丹  王 俊  高海峰  李宁静  吴 伟  刘太国  陈万权  高 利
作者单位:1. 中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193; 2. 新疆维吾尔自治区植物保护站, 乌鲁木齐 830049; 3. 新疆农业科学院植物保护研究所, 乌鲁木齐 830091; 4. 新疆新源县农业技术推广站, 伊犁 835800
基金项目:国家重点研发计划 (2018YFD0200406); 国家自然科学基金国际(组织间)合作项目(31761143011); 国家自然科学基金(31571965)
摘    要:小麦网腥黑穗病是由小麦网腥黑粉菌Tilletia caries引起,会导致小麦严重减产。为了获得小麦网腥黑穗病较高的发病率,本研究利用穗部接菌、土壤接菌、根部接菌3种方法进行温室内人工接种小麦。结果表明,利用穗部接菌及土壤接菌发病率分别为52.5%和10%,而根部接菌发病率仅有4%。利用穗部接菌可以获得更多的发病植株,为更好地研究寄主与病原的互作关系奠定了基础。

关 键 词:小麦网腥黑粉菌  人工接菌  温室  发病率
收稿时间:2019/10/21 0:00:00
修稿时间:2019/12/25 0:00:00

Exploring of the artificial inoculation methods of Tilletia caries
QIN Dandan,WANG Jun,GAO Haifeng,LI Ningjing,WU Wei,LIU Taiguo,CHEN Wanquan,GAO Li.Exploring of the artificial inoculation methods of Tilletia caries[J].Plant Protection,2021,47(1):165-168.
Authors:QIN Dandan  WANG Jun  GAO Haifeng  LI Ningjing  WU Wei  LIU Taiguo  CHEN Wanquan  GAO Li
Institution:1. State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China; 2. Plant Protection Station of Xinjiang Uygur Autonomous Region, Urumqi 830049, China; 3. Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, China; 4. Agricultural Technology Promotion Station of Xinyuan County, Xinjiang, Yili 835800, China
Abstract:Tilletia caries can lead to common bunt of wheat and it can causes severe wheat yield reduction. In order to obtain the diseased plants of T. caries, three methods including tassel inoculation, soil inoculation and root inoculation were used to artificially inoculate in greenhouse. The results showed that disease incidence with tassel inoculation and soil inoculation was 52.5% and 10%, but the disease incidence with root inoculation was only 4%. More diseased plants can be obtained by using tassel inoculation, which lays a foundation for better research on the interaction between the host and the pathogen.
Keywords:Tilletia caries  artificial inoculation  greenhouse  disease incidence
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