L-瓜氨酸对游泳力竭小鼠生长性能及血清生化指标影响的研究

试验通过在饮水中添加不同剂量的L-瓜氨酸（L-Cit），探究其对游泳力竭强应激后，小鼠的生长性能及血清生化指标的影响。选用36只平均体重为（14.58±0.77）g的断奶雌性昆明小鼠，随机分为3组，每组6个重复。对照组饮用自来水，试验Ⅰ组和Ⅱ组在饮水中分别添加0.5%和1.0%的L-Cit。试验期21 d，每3 d空腹称重后在（31±2）℃水桶中负重游泳至力竭，于试验第21天摘眼球采血。结果表明，与对照组相比，试验Ⅰ组、Ⅱ组在第6、9、12天时，雌鼠体重分别提高了12.88%（P < 0.05）、20.30%（P < 0.01）、7.99%（P < 0.05）和21.27%（P < 0.01）、24.47%（P < 0.01）、19.46%（P < 0.01）。第15天时试验Ⅱ组小鼠体重比对照组提高13.71%（P < 0.01）。与对照组相比，试验Ⅰ组小鼠血清中抗超氧阴离子自由基（ASAFR）活力提高了31.49%（P < 0.01）；试验Ⅱ组血清中谷胱甘肽过氧化物酶（GSH-Px）活性和抑制羟自由基（IHFR）能力分别提高54.35%（P < 0.01）和13.10%（P < 0.01）；与对照组和试验Ⅰ组相比，试验Ⅱ组小鼠血清白蛋白（ALB）水平分别提高13.16%（P < 0.01）和6.28%（P < 0.01）。综合试验结果，饮水中添加L-Cit可提高运动后小鼠的生长性能、抗氧化性能及血清总蛋白（TP）和白蛋白含量。综合比较，添加1.0% L-Cit（69.09 mg/d）效果更好。

Study of L-citrulline on Growth Performance of Swimming Exhausted and Serum Biochemical Effect of Mice

Effects of adding different doses of L-citrulline (L-Cit) in drinking water on the growth performance and serum biochemical indexes of swimming exhausted Kunming mice was studied in this paper.36 weaned females with average weight of (14.58±0.77) g were selected and randomly divided into 3 groups with 6 replicates in each group.The mice in control group drank from tap water,and 0.5% and 1% of L-Cit were added in the drinking water for test groupⅠand Ⅱ,respectively.The trial lasted 21 d.Every 3 d,the mice were weighed in an empty stomach and then swam with weight loading in (31±2)℃ bucket until exhausted.Blood was collected from the eyeballs at day 21.The results showed that:Compared with control group,at the day 6,9 and 12,the body weights of the female mice in test groups Ⅰ and Ⅱ increased by 12.88% (P < 0.05),20.30% (P < 0.01),7.99% (P < 0.05) and 21.27% (P < 0.01),24.47% (P < 0.01),19.46% (P < 0.01) respectively.And it increased by 13.71% (P < 0.01) at day 15 in test group Ⅱ.The serum activity of anti superoxide anion free radical (ASAFR) increased by 31.49% (P < 0.01) in test group Ⅰ.The serum activity of GSH-Px and inhibit hydroxyl free radical (IHFR) ability were increased by 54.35%(P < 0.01)and 13.10%(P < 0.01)in test group Ⅱ,respectively.Compared with the control group and test groupⅠ,the serum levels of serum albumin (ALB)content were respectively increased by 13.16% (P < 0.01) and 6.28% (P < 0.01).In conclusion,Adding L-Cit in drinking water could improve the growth performance and antioxidant capacity,increase serum total protein (TP) and albumin contents in mice after exercise,and adding 0.1% L-Cit (69.09 mg/d) was better.