高羊茅FaRVE8基因的克隆、亚细胞定位及表达分析

生物钟是一个复杂的调控网络,在环境的不断变化中促进植物的生长,REVEILLE8 (RVE8)是生物钟的重要基因。为探讨其分子机理,采用RT-PCR和RACE方法,克隆高羊茅叶片FaRVE8基因,结果显示,FaRVE8全长为1881 bp,有1236 bp的开放阅读框,编码411个氨基酸,同属于MYB样因子。遗传进化树表明其与禾本科植物二穗短柄草、节节麦、大麦的同源蛋白亲缘关系较近。利用荧光定量分析高羊茅叶片中FaRVE8在不同光照处理下的表达特征,结果表明,FaRVE8在不同光照处理下均有表达,且呈现出明显的昼夜节律。亚细胞定位显示FaRVE8定位在细胞核中,可能在细胞核中发挥重要作用。以上研究表明,FaRVE8在调节生物节律中有重要作用,为进一步探讨FaRVE8基因的功能和分子调控机制奠定基础。

Cloning,subcellular localization and expression analysis of the RVE8 gene from Festuca arundinacea

The circadian clock is a complex regulatory network that promotes plant growth in the ever-changing environment. REVEILLE8 (RVE8) is an important gene in the circadian clock. In order to explore the molecular mechanism, the FaRVE8 gene of leaves from Festuca arundinacea was cloned by RT-PCR and RACE. It was found that the full length of FaRVE8 was 1881 bp, the open reading frame was 1236 bp, encoding 411 amino acids, which belonged to a MYB-like factor. The phylogenetic analysis showed that it is closely related to Brachypodium distachyon, Aegilops tauschii, and Hordeum vulgare. Real-time fluorescence quantitative analysis of FaRVE8 expression in F. arundinacea leaves under different light treatments showed that FaRVE8 was expressed under different light treatments and showed obvious circadian rhythm. Subcellular localization indicated that FaRVE8 is localized in the nucleus, and FaRVE8 may play an important role in the nucleus. These above studies indicate that the FaRVE8 gene plays an important role in regulating biological rhythms, laying the foundation for further study of the function and molecular regulation mechanisms of the FaRVE8 gene.