几种化学物质对油菜叶片β-1,3-葡聚糖酶活性诱导的研究

摘要：【研究目的】在温室内种植中油杂2号油菜，当长至2-3片真叶时，使用水杨酸（10mg/mL）、核黄素（1mmol/L）、氯化汞（0.01%）和BTH（0.5mmol/L）四种化学物质，对叶片进行诱导处理，研究其对油菜叶片β-1,3-葡聚糖酶的诱导。【结果】水杨酸、核黄素和氯化汞处理的油菜叶片，β-1,3-葡聚糖酶在短期内活性达到第一个高峰后随之下降，然后又上升。BTH处理的油菜叶片在处理后第五天达到活性高峰。【结论】几种化学物质处理后油菜叶片中β-1,3-葡聚糖酶的活性均有不同程度的提高；其中以BTH的诱导作用最强。对于油菜β-1,3-葡聚糖酶活性诱导的研究有利于深入了解β-1,3-葡聚糖酶在病程反应过程中的作用。

Inducing β-1, 3-Glucanase Activities of Rape by Four Chemical Inducers

In the greenhouse, rape leaves were sprayed with four chemical inducers （0.01% mercuric chloride, 10 mg/mL salicylic acid, 1 mmol/L riboflavin, 0.1 mmol/L BTH, respectively） from 2008 to 2009. The leaves treated with water as control. Then, all the leaves were collected and used to extract the β-1, 3-glucanase and to measure the activities. When treated with mercuric chloride, salicylic acid, riboflavin, the β-1, 3-glucanase activity raised in the begin days and declined. However, the β-1, 3-glucanase activity raised to the peak on the six day when treated with BTH. The results showed that the levels of β-1, 3-glucanase activity were all induced when treated with four chemical inducers. Moreover, the BTH has the best effect on induction of the β-1, 3-glucanase.