| 大豆未成熟子叶诱导胚状体发生再生植株 |
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| 引用本文： | 薛仁镐,刘淑兰,韩碧文. 大豆未成熟子叶诱导胚状体发生再生植株[J]. 延边大学农学学报, 1995, 0(3) |
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| 作者姓名： | 薛仁镐 刘淑兰 韩碧文 |
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| 作者单位： | 延边农学院，北京农大生物学院 |
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| 摘 要： | 本文以大豆未成熟子叶为外植体，进行了大豆胚状体发生和再生植株的研究。结果表明：ＭＳ基本培养基附加高浓度的生长素（２．４—Ｄ或ＮＡＡ或２．４—Ｄ与ＮＡＡ的配合），可成功地诱导胚状体发生。２．４—Ｄ比ＮＡＡ具有更强的诱导效果；２．４—Ｄ与ＮＡＡ配比所诱导的正常胚率高于其单用；未成熟子叶不同的放置方式对胚状体发生影响极大。对畸型胚进行了改良并获得了完整小植株。
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| 关 键 词： | 大豆；未成熟子叶；胚状体；植株再生 |
Plant Regeneration From Immature Cotyledons Culture of Soybean Via Somatic Embryogenesis |
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| Xue Rengao et al.. Plant Regeneration From Immature Cotyledons Culture of Soybean Via Somatic Embryogenesis[J]. Journal of Agricultural Science Yanbian University, 1995, 0(3) |
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| Authors: | Xue Rengao et al. |
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| Affiliation: | Yanbian Agricultural College |
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| Abstract: | Embryogenesis from immature cotyledons and plant regeneration of soybean were studied. The resultS showed that embryogenesis from immature cotyledons of soybean was induced successfully by culturing cotyledons on MS medium containing high concentration of 2.4-D or NAA or mixing 2.4-D with NAA. The frequency of normal embryo induced by mixing 2.4-D with NAA was higher than that induced by 2.4-D or NAA. The effects of different placing ways of immature cotyledons on embryogenesis were very remarkable.Abnormal embryo was improved to normal embryo. |
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| Keywords: | Soybean Immature Cotyledon Somatic embryo Plant regeneration |
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