4种野生芍药的花粉形态研究

花粉和孢子，同其他的生殖器官一样，较少受环境的影响（埃尔塔曼，1978；王开发等，1983），其形态属于比较稳定的性状。近些年来，不少学者借助扫描电镜观察园艺植物的花粉形态，阐释了许多园艺植物种间或品种间的亲缘关系（张秀英等，1997；吕华飞等，1994；袁涛等，1999）。关于芍药（Paeonia）的该项研究，报道很少（席以珍，1984）。本文以采自野外的4个种（居群）的花粉为主要研究材料，建立了芍药花粉形态的量化指标。

Study on Pollen Morphology of 4 Wild Herbaceous Peony

In order to discuss the relationship among the wild herbaceous peonies native in China according to microscopic characteristics, the pollen grains of four wild herbaceous peonies were examined under scanning electron microscope (SEM), and the data acquired were used in clustering analysis. The result with SEM showed distinctly distinguishing extine surface pattern of taxonomic importance among the four species: reticulate pattern for Paeonia lactiflora; micro-foveolate, regulate and regulate-foveolate pattern for P. veitchii ; regulate and subcerebro-reticulate pattern for P. obovata ; regulate pattern for P. anomala var. intermedia . The result with clustering analysis suggested the close relationship between P. veitchii and P. anomala var. intermedia for their Euclidean distance was only 2.51, and the alienation between P. lactiflora and the other three species for their Euclidean distances were all more than 5.00. In addition, we deduced P. obovata may be the linkage between subsect Foliolatae and subsect Dissectifoliae for its pollen morphology was appreciably inclined to P. veitchii and P. anomala var. intermedia.