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Sample type is vital for diagnosing infection with peste des petits ruminants virus by reverse transcription PCR
Authors:Pam Dachung Luka   Chrisostom Ayebazibwe   David Shamaki   Frank Norbert Mwiine   Joseph Erume
Abstract:Peste des petits ruminants (PPR) diagnosis from suspected samples from sheep and goats was carried out. Buffy coat, tissues, and oculo-nasal swabs were analyzed using nucleoprotein (NP3/NP4) and fusion protein (F1/F2) gene primers, respectively. Analysis of the sample types and primer set revealed that buffy coat are the best type of samples for PPR diagnosis and the use of two set of primers will increase the number of positives.
Keywords:diagnosis   goats   peste des petits ruminants   sample   sheep
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