单克隆抗体-胶体金标记技术快速检测柑桔黄龙病

应用单克隆抗体-胶体金标记技术，采用徒手切片法和斑点试验法检测柑桔黄龙病获得成功。该方法检测速度快，操作简单，成本低，适合柑桔种苗大批量检测和田间普查。采用单抗金标技术对田间栽培的柑桔品种、柑桔实生苗、试管微芽嫁接苗、热处理脱毒后的嫁接苗进行检测，结果表明田间栽培的6个柑桔品种不论是否显示黄龙病症状均检测到柑桔黄龙病病菌，而消毒种子播种所得实生苗及试管微芽嫁接苗为无病株，带病株经过30d 40oC恒温热处理后产生的新梢及其嫁接苗绝大部分也是无病株。

Rapid Detection of Citrus Huang-Long-Bing Pathogen by Monocolony Antibody Colloidial Gold Labelled Technique

The research had succeeded in detecting the Citrus Huanglongbing through the hand-sliced method or dot-marked observation by the monoclonal antibody with colloidal gold-labeled (MACG) technique. The methods are suitable for Citrus seedling detection of Huanglongbing pathogen in batch and general investigation in field, for they are rapidly and easily manipulated and low in price. By the MACG technique, we detected the Citrus plants in field, Citrus seedlings, their microbud-grafting plantlets and the plants after heat-therapy processed, the result showed that 6 kinds of Citrus plant in field, no matter the symptom of Huanglongbing pathogen was appeared or not, were detected to be infected by Huanglongbing pathogen. However microbud-grafting plantlets in tube and the seedlings, which grew from sterilized Citrus seeds, were Huanglongbing pathogen-free. Even most of the new branches and theirs-grafted plants which came from plants-infected after stable temperature processed for 30 days in 40℃ had no Huanglongbing pathogen.