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| 单核细胞增生性李斯特菌分离株的生物学特性研究 | |
| 引用本文： | 吴晓薇,徐成刚,张朝冠,江经纬,叶贺佳,区燕宜,徐小芹,廖明.单核细胞增生性李斯特菌分离株的生物学特性研究[J].中国兽医杂志,2012,48(7):11-13. |
| 作者姓名： | 吴晓薇 徐成刚 张朝冠 江经纬 叶贺佳 区燕宜 徐小芹 廖明 |
| 作者单位： | 1. 华南农业大学兽医学院,广东广州510642;广东检验检疫技术中心,广东广州510623 2. 华南农业大学兽医学院,广东广州510642;广东省动物源性人兽共患病预防与控制重点实验室,广东广州510642 3. 南海出入境检验检疫局,广东佛山,528200 4. 华南农业大学兽医学院,广东广州,510642 |
| 基金项目： | 广东出入境检验检验局科技项目 |
| 摘 要： | 对从广州禽产品分离的6株LM进行了药敏试验、pH值的适应范围、动物致病性等生物学特性研究。6株LM分离株对先锋必、氯霉素、卡那霉素、头孢唑啉、庆大霉素等多种药物敏感;而对多黏菌素B、复方新诺明、新生霉素有耐药性。LM分离株在pH值4.0～10.5范围内可存活。以109 CFU剂量感染SPF小鼠、清洁级新西兰兔和健康粤黄鸡,小鼠的致死率达100%,可引起兔结膜炎等症状,但未引起试验鸡的任何不适。6株LM分离株的Hly基因与标准参考株基因相似性达到96.1%以上,说明该基因在LM中较为保守。 |
| 关 键 词： | 单核细胞增生性李斯特菌 禽产品 生物学特性 Hly基因 |
Characterization of 6 Listeria Monocytogene isolates obtained from Guangzhou poultry products |
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| WU Xiao-wei , XU Cheng-gang , ZHANG Chao-guan , JIANG Jing-wei , YE He-jia , OU Yan-yi , XU Xiao-qin , LIAO Ming.Characterization of 6 Listeria Monocytogene isolates obtained from Guangzhou poultry products[J].Chinese Journal of Veterinary Medicine,2012,48(7):11-13. | |
| Authors: | WU Xiao-wei XU Cheng-gang ZHANG Chao-guan JIANG Jing-wei YE He-jia OU Yan-yi XU Xiao-qin LIAO Ming |
| Institution: | 1,3(1.College of Veterinary Medicine,South China Agricultural University,Guangzhou,510642,China; 2.Guangdong Entry-Exit Inspection and Quarantine Technique Center,Guangzhou 510623,China; 3.Guangdong Key Lab of Prevention and Control of Zoonosis,Guangzhou 510642,China; 4.Nanhai Entry-Exit Inspection and Quarantine Bureau,Foshan 528200,China) |
| Abstract: | Six Listeria Monocytogene isolates were obtained from poultry products in Guangzhou.Anti-microbial resistance,suitable growth pH range and pathogenicity of the isolate were examined.All 6 isolates were sensitive to many drugs including Cefoperazone Sodium,Chloramphenicol,Kanamycin,Cefazolin V,Gentamicin;resistant to Polymyxin B,trimethoprim-sulfamethoxazole,Novobiocin.The isolates could survive in pH 4.0~10.5,and could cause BABL/c mice death with 109 CFU dose by intraperitoneal infection.The same dose of the isolates could cause conjunctivitis in New Zeeland rabbits by injection via eye-drop route.The same dose to healthy Guangdong Huang chickens by intramuscular injection did not cause clinical signs.These hly gene nucleotide sequences had a high homology of 96.1%~100% with C53005,C53003 and DQ054589,DQ844148,EF182456 in GenBank database,so hly gene was conserved in LM. |
| Keywords: | Listeria Monocytognes Biological characteristic Hly |
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