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| 枯草芽孢杆菌D-29菌株发酵条件的优化 | |
| 引用本文： | 周芳,谈静惠,王美琴,李新凤,郝晓娟. 枯草芽孢杆菌D-29菌株发酵条件的优化[J]. 山西农业科学, 2013, 0(12): 1333-1335 |
| 作者姓名： | 周芳 谈静惠 王美琴 李新凤 郝晓娟 |
| 作者单位： | 山西农业大学农学院,山西太谷030801 |
| 基金项目： | 国家自然科学基金青年基金项目（31000873）;山西省科技攻关项目（20120311016-2）;山西农业大学中青年学术骨干基金项目（XG201210） |
| 摘 要： | 枯草芽孢杆菌D-29是一株具有广谱抑菌活性的生防菌株。对D-29菌株发酵条件进行了优化,结果表明,最佳初始培养基配方为:玉米粉10 g,麦芽糖30 g,碳酸钙1.5 g,豆粉30 g,水1 000 mL;最佳培养条件为:250 mL三角瓶装液量100 mL,初始pH值为7,接菌量1%,培养温度28℃,培养时间48 h,转速170190 r/min。 |
| 关 键 词： | 枯草芽孢杆菌 发酵条件 生物防治 |
Optimization of Fermetation Conditions of Bacillus subtilis Strain D-29 |
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| ZHOU Fang,TAN Jing-hui,WANG Mei-qin,LI Xin-feng,HAO Xiao-juan. Optimization of Fermetation Conditions of Bacillus subtilis Strain D-29[J]. Journal of Shanxi Agricultural Sciences, 2013, 0(12): 1333-1335 | |
| Authors: | ZHOU Fang TAN Jing-hui WANG Mei-qin LI Xin-feng HAO Xiao-juan |
| Affiliation: | (College of Agronomy, Shanxi Agricultural University, Taigu 030801, China) |
| Abstract: | Abstract：Bacillus subtilis D-29 is an antagonistic bacterium with broad-spectrum antimicrobial activity. The optimal for culture media and fermentation conditions of D-29 were determined in the study. Results showed that optimum fermentation nL component for strain Bacillus subtilis D-29 was corn flour 10 g, maltose 30 g, soybean dregs 30 g, CaC03 1.5 g, and water I 000 mt obtained best fermentation condition was described as： amount of load 100 mL （in a 250 mL flask）, amount of inoculation 1%, bt pH 7, the fermentation temperature 28 ℃, incubation time 48 h, and rotation speed 170-190 r/min. |
| Keywords: | Bacillus subtilis fermetation conditions biological control |
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