富士系苹果过氧化物酶同工酶研究

以24个富士系苹果品种为试材,提取其叶片和叶柄的酶液,利用聚丙烯酰胺凝胶电泳,研究其过氧化物酶同工酶谱的特征,结果表明:富士系苹果叶片的过氧化物酶同工酶酶谱有11条酶带,部分品种间酶带数量及强弱不同,Rf值为0.09~0.41。叶柄有11条酶带,除了秋富1比其他品种多了Rf0.46带外,每个品种都具有其他10条谱带,叶柄的过氧化物酶同工酶酶谱Rf值为0.07~0.46。叶片和叶柄的酶谱带有差异,叶片具有Rf0.09,Rf0.11和Rf0.21共3条特征带;叶柄具有Rf0.07,Rf0.32的特征带,Rf0.46为秋富1叶柄所特有的谱带。叶片过氧化物酶谱相似系数在0.71~1.0之间。通过聚类分析,可以将以上品种分成8个类群,能直接鉴定优良短枝富士、寿富士、秋富1号和烟富3号等4个品种,另外各类群之间的品种也可相互鉴定。

Peroxidase Isozyme in Some Strains of Fuji Apple

With 24 strains of Fuji apple as materials,through extracting enzyme from leaves and leafstalks,the peroxiase isozyme of Fuji apple was studied.There were 11 peroxiase isozyme belts in leaves of Fuji apple strains.The amount and activity of the belts were different among some strains.Rf ranged from 0.09 to 0.41.There were 11 peroxiase isozyme belts in leafstalks.Only Qiufu1 had Rf 0.46 map,all strains possessed the other 10 belts.The Rf ranged from 0.07 to 0.46.There were differences in belts between leaves and leafstalks.Leaves had three characteristic belts Rf 0.09,Rf 0.11 and Rf 0.21.Leafstalks had Rf 0.07 and Rf 0.32 characteristic belts.Rf 0.46 was for Qiufu1.The similar coefficients of leaves peroxiase isozyme belts between stains were between 0.71 to 1.Through cluster analysis,the strains were divided into 8 groups.It could identify four strains of Elitefuji,Shou Fuji,Qiufu1 and Yanfu3.The strains in different groups could also be identified.