| 青蒿组织中快速有效DNA提取方法的研究 |
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| 引用本文： | 石开明,周毅峰.青蒿组织中快速有效DNA提取方法的研究[J].农业科学与技术,2008,9(2):36-38. |
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| 作者姓名： | 石开明 周毅峰 |
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| 作者单位： | [1]湖北民族学院生物科学与技术学院,湖北恩施445000 [2]湖北省生物资源保护与利用重点实验室,湖北恩施445000 [3]湖北大学中药生物技术省重点实验室,湖北武汉430062 |
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| 基金项目： | 生物资源保护与利用湖北省重点实验室(湖北民族学院)资助项目，湖北大学校科研和教改项目，湖北民族学院院内项目 |
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| 摘 要： | 传统的CTAB法成本高、毒性较大且操作繁琐，往往仅在需DNA量较大的RFLP等分析中采用。SDS提取的DNA产率接近CTAB产率的2倍，但纯度、完整性不如后者。常用的植物DNA简易提取方法有碱处理法及高温水煮法等。这些简易提取方法大都速度快、成本低，但提取DNA质量差、保存时间短、不适于PCR扩增。该文以SDS法为依据，在此基础上比较了3种方法的提取效果，从而摸索出一种快速有效的提取青蒿DNA的方法（以下简称“剪碎法”）。
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| 关 键 词： | 青蒿 组织 DNA提取方法 CTAB法 剪碎法 |
An Improved Method of Extracting Artemisia abrotanum Genomic DNA |
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| SHI Kai-ming,ZHOU Yi-feng.An Improved Method of Extracting Artemisia abrotanum Genomic DNA[J].Agricultural Science ＆ Technology,2008,9(2):36-38. |
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| Authors: | SHI Kai-ming ZHOU Yi-feng |
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| Institution: | SHI Kai-ming, ZHOU Yi-feng (1. College of Biology Science and Technology ,Hubei Institute for Nationalities, Enshi 445000 ; 2. Key Laboratory of Biological Resource Protection and Utilization in Hubei Province, Enshi 445000; 3. Key Laboratory of Biotechnology in Traditional Chinese Medicine of Hubei Province, Hubei University, Wuhan 430062) |
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| Abstract: | Objective] The objective of this study is to explore a rapid and efficient method of extracting gonomic DNA from Artemisia abrotanum. Method] Three methods of Cutting Method, Liquid Nitrogen Method and Quartz Sand Method based on SDS method were employed to extract Artemisia abrotanum genomic DNA from tender leaf at seedling stage, tender spike and old leaf at heading stage. The obtained DNAs were detected by ahsorbance de-tection, agarose gel and PCR amplification. Result] Cutting Method performed better than the other two methods compared in purity, extracting cycle and cost, accordingly more suitable for PCR amplification. The results also show that young spike is the best material for extracting genomic DNA flora Artemis-ia Annua. |
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| Keywords: | Artemisia abrotanum DNA extraction Cutting method |
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