小麦TaKu70和TaKu80基因的克隆和分析

Ku蛋白与动植物细胞DNA损伤修复和辐射敏感性有着密切联系，其功能及表达正常与否，关系到细胞DNA双链断裂（DSBs）的修复能力。为了研究Ku蛋白在小麦DNA损伤修复和对辐射敏感性中的作用，本实验室克隆了完整的小麦Ku70和Ku80 cDNA 序列，分别命名为TaKu70和TaKu80。小麦TaKu70和TaKu80分别编码626个和706个氨基酸残基的Ku70/Ku80蛋白序列。多重序列分析表明小麦Ku70蛋白与水稻和拟南芥Ku70蛋白的同源性分别为94%和78%；小麦Ku80蛋白与水稻和拟南芥Ku80蛋白的同源性分别为85%和69%。结构域和功能分析表明小麦Ku70/Ku80蛋白含有Ku蛋白的核心组分——Ku70/ Ku80-core结构域，以及只有真核生物Ku蛋白才有的vWA 结构域。

Cloning of TaKu70 and TaKu80 in Wheat

Ku protein participates in DNA damage repair and responses to irradiation in both animal and plant cells. Its function and expression closely related to the ability of DSBs repair of cells. To study the Ku protein in wheat, we cloned the complete cDNA sequence of wheat Ku70 and Ku80 ,named TaKu70 and TaKu80 , respectively. TaKu70 /TaKu80 codes wheat Ku70/Ku80 protein, with 626 and 706 amino acid residues, respectively. Multiple sequence analysis showed that the protein amino acid sequences that were coded by wheat TaKu70 have 94% and 78% homology with Ku70 protein in rice and Arabidopsis respectively; The protein amino acid sequences that were coded by wheat TaKu80 had 85% and 69% homology with Ku80 protein in rice and Arabidopsis respectively. Domain and functional analysis showed that the wheat Ku protein contained the core component - Ku70 / Ku80-core domain, and the vWA domain ,which was only exist in the eukaryotic cell.