| 驱蚊香草的组织培养技术 |
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| 引用本文： | 李际红,周庆和,谢会成.驱蚊香草的组织培养技术[J].中南林业科技大学学报,2005,25(5):92-96. |
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| 作者姓名： | 李际红 周庆和 谢会成 |
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| 作者单位： | 山东农业大学科技学院，山东农业大学科技学院，山东农业大学科技学院 山东 泰安 271000，山东 泰安 271000，山东 泰安 271000 |
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| 摘 要： | 利用驱蚊香草茎段进行组织培养,研究了添加在培养基中不同激素组成、浓度、培养条件等因素对不定芽诱导、继代增殖、防止玻璃化以及生根的影响.结果表明:适于驱蚊香草不定芽诱导的培养基为MS 6-BA1.5 mg/L NAA0.05 mg/L,适于继代增值的培养基为MS 6-BA1.5 mg/L NAA0.05mg/L,适于生根的培养基为配方为1/2MS IBA0.5mg/L NAA0.1 mg/L;用透气设施的封口材料明显地降低驱蚊香草的玻璃化;基质培养基生根优于琼脂培养基.
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| 关 键 词： | 生物技术 驱蚊香草 组织培养 不定芽 玻璃化 封口材料 |
| 文章编号： | 1000-2502(2005)05-0092-05 |
| 修稿时间： | 2005年2月2日 |
Studies of the Tissue Culture Techniques of Citrosa |
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| LI Ji-hong,ZHOU Qing-he,XIE Hui-cheng.Studies of the Tissue Culture Techniques of Citrosa[J].Journal of Central South Forestry University,2005,25(5):92-96. |
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| Authors: | LI Ji-hong ZHOU Qing-he XIE Hui-cheng |
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| Abstract: | Taking the sprout explants of Citrosa for tissue culture, this paper makes a study of the influences of the factors such as different hormone composition, concentration and culture conditions in culture medium upon adventitious bud induction, shoot proliferation, anti-vitrification and rooting. The results show that the optimum medium for adventitious bud regeneration is MS+6-BA1.5 mg/L+NAA0.05 mg/L; the suitable culture medium for shoot proliferation is MS+6-BA1.5 mg/L+NAA0.05 rag/L; and the proper culture medium of rooting is 1/2MS+IBA0.5 mg/L+NAA0.1 mg/L; and that to reduce vitrification in culture, the use of membrane filter gets a higher ratio in rooting than the use of other sealing materials. |
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| Keywords: | biotechnology Citrosa tissue culture adventitious bud vitrification sealing material |
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