免疫分析中五参数Logistic模型的应用

在计算机中用五参数 Logistic 回归方程对玉米赤霉烯酮不同免疫分析方法的数据进行处理,方便、快速、准确地建立标准曲线及测定样品中未知抗原浓度,克服了手工绘制曲线、查算抗原浓度的随意性及繁琐性,使免疫分析方法的数据处理更科学更简便,同时与常用的 Logit-log 模型的处理结果进行了比较,表明五参数 Logistic 回归模型更合理更准确。模型中的五个参数具有特定的物理及生物意义,与免疫分析方法的基本参数有一定的联系。

Application of the 5-parameter Logistic Model in Immunoassay

The data of different immunoassay for zearalenone was processed by 5-parameter logistic regression function with computer.It was fast,accurate and convenient to establish standard curve and to measure contents of antigen in sample,and hence,the random and tedious processing by hand were over- come.Meanwhile,compared to logit-log model,the results of 5-parameter logistic model was more ratio- nal and accurate.The five parameters in logistic regression function possesses special physica and biologi- cal signi...