准噶尔山楂叶总黄酮的超声波提取及抗氧化研究

目的]为准噶尔山楂叶的综合利用和深度开发提供一定的参考。方法]利用超声波法提取准噶尔山楂叶中的总黄酮,用总黄酮提取率作为衡量提取工艺的指标,对影响超声波提取的多个因子进行单因素、正交试验分析。结果]准噶尔山楂叶中的总黄酮的超声波提取最佳条件为:乙醇浓度80%,提取温度50℃,提取时间50 min,提取功率75 W,总黄酮的得率高达84.50%;影响总黄酮得率的主次因素依次为:乙醇浓度提取时间提取功率提取温度。结论]准噶尔山楂叶总黄酮有较强的清除自由基能力,表现出明显的抗氧化性。

Study on the Ultrasonic Extraction and Antioxidative Activity of Total Flavonoids from Crataegus songorica K. Koch.Leaves

Objective] To provide references for the comprehensive utilization and deep processing of Crataegus songorica K.Koch leaves.Method] The total flavonoids were extracted from Crataegus songorica K.Koch leaves by ultrasonic wave,and the impact factors were analyzed by single factor and orthogonal experiments.Result] The best ultrasonic extraction conditions were 80% ethanol concentration,50 ℃ extraction temperature,50 min extraction time and 75 W extraction power,under the conditions,the yield of total flavonoids could reach up to 84.50%,and the influential degree of each impact factor decreased in the order of ethanol concentration > ultrasonic time >ultrasound power >ultrasonic temperature.Conclusion] The total flavonoids from Crataegus songorica K.Koch leaves had strong radical scavenging ability and significant antioxidative activity.