水法提取普洱茶茶多糖条件优化

目的]筛选热水浸提法提取普洱茶中水溶性茶多糖的最佳条件。方法]采用热水浸提法提取普洱茶中的水溶性茶多糖,根据浸提时间、浸提温度、料液比、提取次数对多糖得率的影响,通过正交试验得到普洱茶水溶性多糖浸提工艺优选因素组合。结果]影响普洱茶茶多糖得率的因素主次顺序为浸提时间提取次数浸提温度料液比;最佳浸提条件为固液比1∶20 g/ml、温度90℃、时间1.5 h、浸提2次;在最佳浸提工艺条件下进行浸提试验,得到的最佳提取率是54.5%。结论]为普洱茶中茶多糖的提取提供了依据。

Optimization of Water Extraction for Tea Polysaccharide from Pu'er Tea

Objective] The aim was to screen the optimum conditions for extracting water-soluble tea polysaccharide from Pu’er tea by hot water extraction method.Method] By using hot water extraction method to extract water-soluble tea polysaccharide,according to the effects of extraction time,temperature,solid-liquid ratio,times on polysaccharide yield,the optimum factors were obtained through orthogonal test.Result] The order of influencing factors for tea polysaccharide extraction from Pu’er tea are extraction time > extraction times > extraction temperature > solid-liquid ratio;the optimum extraction conditions are solid-liquid ratio 1∶20 g/ml,temperature 90 ℃,time 1.5 h,extraction 2 times;under the above conditions,the optimum extraction rate is 54.5%.Conclusion] The study will provide basis for tea polysaccharide extraction from Pu’er tea.