| 紫外分光光度法测定金花葵籽中总黄酮的含量 |
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| 引用本文： | 危晴,兰蓉,辛秀兰,陈亮.紫外分光光度法测定金花葵籽中总黄酮的含量[J].安徽农业科学,2012(12):7050+7060. |
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| 作者姓名： | 危晴 兰蓉 辛秀兰 陈亮 |
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| 作者单位： | 1. 北京电子科技职业学院,北京,100029
2. 北京化工大学生命科学与技术学院,北京,100029 |
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| 基金项目： | 北京市属市管高等学校人才强教深化计划项目资助 |
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| 摘 要： | 目的]建立金花葵籽中总黄酮含量的测定方法。方法]采用紫外分光光度法测定金花葵籽总黄酮含量。结果]采用紫外分光光度法测定总黄酮含量时,在0.005～0.025 mg/ml有良好的线性关系(R=0.999 9),平均回收率为100.4%,RSD为0.016%;金花葵籽中总黄酮的含量为2.08。结论]所用方法简便、准确,可用于金花葵籽的质量控制。
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| 关 键 词： | 金花葵籽 总黄酮 含量测定 紫外分光光度法 |
Determination of Total Flavonoids Content in Golden Kwai Seed by Ultraviolet Spectrophotometry |
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| Institution: | WEI Qing et al(Beijing Polytechnic,Beijing 100029) |
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| Abstract: | Objective] The aim was to establish the method for determining total flavonoids content in Golden Kwai seed.Method] UV spectrophotometry was adopted to determine the content of total flavonoids.Result] The results showed that in the range of 0.005-0.025 mg/ml,the excellent 1inear relationship was obtained with the relative coefficient of 0.999 9,the average recovery rate of 100.4%,the RSD of 0.016%;total flavonoids content in Golden Kwai seed was 2.08.Conclusion] The method is simple and reliable,which could be used to control the quality of Golden Kwai seed. |
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| Keywords: | Golden Kwai seed Total flavonoids Content determination Ultraviolet spectrophotometry |
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